Production of minicellulosomes from Clostridium cellulovorans for the fermentation of cellulosic ethanol using engineered recombinant Saccharomyces cerevisiae

被引:30
作者
Hyeon, Jeong-eun [1 ]
Yu, Kyung-Ok [1 ]
Suh, Dong Jin [2 ]
Suh, Young-Woong [2 ]
Lee, Sung Eun [3 ]
Lee, Jinwon [4 ]
Han, Sung Ok [1 ]
机构
[1] Korea Univ, Sch Life Sci & Biotechnol, Seoul 136701, South Korea
[2] Korea Inst Sci & Technol, Clean Energy Res Ctr, Seoul, South Korea
[3] Nanotoxtech Inc, Res Stn, Ansan, Gyeonggi Do, South Korea
[4] Sogang Univ, Dept Chem & Biomol Engn, Seoul, South Korea
关键词
minicellulosome; Clostridium cellulovorans; Saccharomyces cerevisiae; cellulose-binding domain; consolidated bioprocessing; ethanol fermentation; CELL-SURFACE; AMORPHOUS CELLULOSE; CRYSTALLINE CELLULOSE; TRICHODERMA-REESEI; BACILLUS-SUBTILIS; BETA-GLUCOSIDASE; DEGRADATION; ENZYMES; YEAST; EXPRESSION;
D O I
10.1111/j.1574-6968.2010.02035.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Saccharomyces cerevisiae was engineered for assembly of minicellulosomes by heterologous expression of a recombinant scaffolding protein from Clostridium cellulovorans and a chimeric endoglucanase E from Clostridium thermocellum. The chimeric endoglucanase E fused with the dockerin domain of endoglucanase B from C. cellulovorans was assembled with the recombinant scaffolding protein. The resulting strain was able to ferment amorphous cellulose [carboxymethyl-cellulose (CMC)] into ethanol with the aid of beta-glucosidase 1 produced from Saccharomycopsis fibuligera. The minicellulosomes assembled in vivo retained the synergistic effect for cellulose hydrolysis. The minicellulosomes containing the cellulose-binding domain were purified by crystalline cellulose affinity in a single step. In the fermentation test at 10 g L-1 initial CMC, approximately 3.45 g L-1 ethanol was produced after 16 h. The yield (in grams of ethanol produced per substrate) was 0.34 g g-1 from CMC. This result indicates that a one-step processing of cellulosic biomass in a consolidated bioprocessing configuration is technically feasible by recombinant yeast cells expressing functional minicellulosomes.
引用
收藏
页码:39 / 47
页数:9
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