Determination of selected neurotransmitter metabolites using monolithic column chromatography coupled with chemiluminescence detection

被引：47

作者：

Adcock, JL ^{[1
]}

Barnett, NW ^{[1
]}

Costin, JW ^{[1
]}

Francis, PS ^{[1
]}

Lewis, SW ^{[1
]}

机构：

[1] Deakin Univ, Sch Biol & Chem Sci, Geelong, Vic 3217, Australia

来源：

TALANTA | 2005年 / 67卷 / 03期

关键词：

neurotransmitter metabolites; monolithic column chromatography; chemiluminescence detection;

D O I：

10.1016/j.talanta.2005.03.007

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The oxidation of selected clinically important neurotransmitter metabolites with acidic potassium permanganate in the presence of polyphosphates evokes chemiluminescence of sufficient intensity to enable the sensitive determination of these species. Limits of detection for 5-hydroxyindole-3-acetic acid (5-HIAA), vanilmandelic acid (VMA; alpha 4-dihydroxy-3-methoxybenzeneacetic acid), 4-hydroxy3-methoxyphenylglycol (MHPG), homovanillic acid (HVA, 4-hydroxy-3-methoxyphenylacetic acid) and 3,4-dihydroxyphenylacetic acid (DOPAC) were between 5 x 10(-9) and 4 x 10(-8) M, using flow-injection analysis methodology. In addition, we demonstrate the rapid determination of homovanillic acid and 5-hydroxyindole-3-acetic acid in human urine - without the need for extraction procedures - using monolithic column chromatography with chemiluminescence detection. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：585 / 589

页数：5

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