Backbone dynamics and amide proton exchange at the two sides of the eremomycin dimer by 15N NMR

被引：10

作者：

Batta, G

Sztaricskai, F

Makarova, MO

Gladkikh, EG

Pogozheva, VV

Berdnikova, TF

机构：

[1] Hungarian Acad Sci, Antibiot Res Grp, H-4010 Debrecen, Hungary

[2] Russian Acad Med Sci, Gause Inst New Antibiot, Moscow 119867, Russia

来源：

CHEMICAL COMMUNICATIONS | 2001年 / 05期

关键词：

D O I：

10.1039/b010144n

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

N-15-labelling of the glycopeptide antibiotic eremomycin has made possible the dynamic characterization of the two sides of the dimer; in the presence of acetate anion the backbone amides are dynamically equivalent and the fastest amide proton exchange rate was found at the heart of the binding pocket.

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收藏

页码：501 / 502

页数：2

相关论文

共 10 条

[1] Measurement of the different affinities of the two halves of glycopeptide dimers for acetate [J].

Bardsley, B ;

Williams, DH .

CHEMICAL COMMUNICATIONS, 1997, (11) :1049-1050

[2] AN NMR-STUDY OF EREMOMYCIN AND ITS DERIVATIVES FULL H-1 AND C-13 ASSIGNMENT, MOTIONAL BEHAVIOR, DIMERIZATION AND COMPLEXATION WITH AC-D-ALA-D-ALA [J].

BATTA, G ;

SZTARICSKAI, F ;

KOVER, KE ;

RUDEL, C ;

BERDNIKOVA, TF .

JOURNAL OF ANTIBIOTICS, 1991, 44 (11) :1208-1221

[3] EREMOMYCIN - NEW GLYCOPEPTIDE ANTIBIOTIC - CHEMICAL-PROPERTIES AND STRUCTURE [J].

GAUSE, GF ;

BRAZHNIKOVA, MG ;

LOMAKINA, NN ;

BERDNIKOVA, TF ;

FEDOROVA, GB ;

TOKAREVA, NL ;

BORISOVA, VN ;

BATTA, GY .

JOURNAL OF ANTIBIOTICS, 1989, 42 (12) :1790-1799

[4] EXPRESSION OF ELECTROSTATIC BINDING COOPERATIVITY IN THE RECOGNITION OF CELL-WALL PEPTIDE ANALOGS BY VANCOMYCIN GROUP ANTIBIOTICS [J].

GROVES, P ;

SEARLE, MS ;

WESTWELL, MS ;

WILLIAMS, DH .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1994, (13) :1519-1520

[5] THE STRUCTURE OF AN ASYMMETRIC DIMER RELEVANT TO THE MODE OF ACTION OF THE GLYCOPEPTIDE ANTIBIOTICS [J].

GROVES, P ;

SEARLE, MS ;

MACKAY, JP ;

WILLIAMS, DH .

STRUCTURE, 1994, 2 (08) :747-754

[6] A N-15 NMR-STUDY OF INTERMOLECULAR INTERACTIONS BETWEEN VANCOMYCIN AND AC-D-ALA-D-ALA [J].

HAWKES, GE ;

MOLINARI, H ;

SINGH, S ;

LIAN, LY .

JOURNAL OF MAGNETIC RESONANCE, 1987, 74 (01) :188-192

[7] MODEL-FREE APPROACH TO THE INTERPRETATION OF NUCLEAR MAGNETIC-RESONANCE RELAXATION IN MACROMOLECULES .1. THEORY AND RANGE OF VALIDITY [J].

LIPARI, G ;

SZABO, A .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1982, 104 (17) :4546-4559

[8] CONFORMATION OF A82846B, A GLYCOPEPTIDE ANTIBIOTIC, COMPLEXED WITH ITS CELL-WALL FRAGMENT - AN ASYMMETRIC HOMODIMER DETERMINED USING NMR-SPECTROSCOPY [J].

PROWSE, WG ;

KLINE, AD ;

SKELTON, MA ;

LONCHARICH, RJ .

BIOCHEMISTRY, 1995, 34 (29) :9632-9644

[9] Heteronuclear multidimensional NMR experiments for the structure determination of proteins in solution employing pulsed field gradients [J].

Sattler, M ;

Schleucher, J ;

Griesinger, C .

PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY, 1999, 34 (02) :93-158

[10] Crystal structure of vancomycin [J].

Schafer, M ;

Schneider, TR ;

Sheldrick, GM .

STRUCTURE, 1996, 4 (12) :1509-1515