Comment on "Unbiased Statistical Analysis for Multi-Stage Proteomic Search Strategies"

被引：29

作者：

Bern, Marshall ^{[1
]}

Kil, Yong J. ^{[1
]}

机构：

[1] Palo Alto Res Ctr, Palo Alto, CA 94304 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2011年 / 10卷 / 04期

关键词：

proteomics; mass spectrometry; peptide identification; bioinformatics; X!Tandem; Paragon; TANDEM MASS-SPECTRA; DATABASE SEARCH; PROTEIN IDENTIFICATIONS; SPECTROMETRY; ALGORITHM; PEPTIDES;

D O I：

10.1021/pr101143m

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Everett et al. recently reported on a statistical bias that arises in the target-decoy approach to false discovery rate estimation in two-pass protcomics search strategies as exemplified by X!Tandem. This bias can cause serious underestimation of the false discovery rate. We argue here that the "unbiased" solution proposed by Everett et al., however, is also biased and under certain circumstances can also result in a serious underestimate of the FDR, especially at the protein level.

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收藏

页码：2123 / 2127

页数：5

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