Tyrosinase biosensor based on zinc oxide nanorods

被引:17
作者
Chen, L. Y. [1 ]
Gu, B. X. [2 ]
Zhu, G. P. [2 ]
Wu, Y. F. [1 ]
Liu, S. Q. [1 ]
Xu, C. X. [2 ]
机构
[1] Southeast Univ, Sch Chem & Chem Engn, Nanjing 211189, Peoples R China
[2] Southeast Univ, Adv Photon Ctr, Sch Elect Sci & Engn, Nanjing 210096, Peoples R China
关键词
ZnO nanorod; tyrosinase; biosensor; phenol; amperommetry;
D O I
10.1142/S1793292007000647
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
A phenol biosensor based on the skillful immobilization of tyrosinase on zinc oxide (ZnO) nanorods was proposed. The ZnO nanorods fabricated by a simple vapor-phase transport method possess a high aspect ratio, good electron communication, chemical purity, smooth and positive charged surface and are ready for immobilization of biochemicals with low isoelectric point (IEP). Electrochemical measurement and Scanning Electron Microscopic (SEM) analysis showed that the enzyme of tyrosinase with IEP 4.5 can be adsorbed on the surface of ZnO nanorods and kept its bioactivity of the oxidation of phenol to a large extent. This led us to develop phenol biosensor with good stability and reproducibility. The proposed method creates a new way to develop biosensors using nanostructured materials with high IEP.
引用
收藏
页码:281 / 284
页数:4
相关论文
共 16 条
  • [1] Conversion of zinc oxide nanobelts into superlattice-structured nanohelices
    Gao, PX
    Ding, Y
    Mai, WJ
    Hughes, WL
    Lao, CS
    Wang, ZL
    [J]. SCIENCE, 2005, 309 (5741) : 1700 - 1704
  • [2] Room-temperature ultraviolet nanowire nanolasers
    Huang, MH
    Mao, S
    Feick, H
    Yan, HQ
    Wu, YY
    Kind, H
    Weber, E
    Russo, R
    Yang, PD
    [J]. SCIENCE, 2001, 292 (5523) : 1897 - 1899
  • [3] ROTATING-RING-DISK ENZYME ELECTRODE FOR BIOCATALYSIS KINETIC-STUDIES AND CHARACTERIZATION OF THE IMMOBILIZED ENZYME LAYER
    KAMIN, RA
    WILSON, GS
    [J]. ANALYTICAL CHEMISTRY, 1980, 52 (08) : 1198 - 1205
  • [4] Renewable phenol biosensor based on a tyrosinase-colloidal gold modified carbon paste electrode
    Liu, SQ
    Yu, JH
    Ju, HX
    [J]. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2003, 540 : 61 - 67
  • [5] Amperometric biosensing of carbamate and organophosphate pesticides utilizing screen-printed tyrosinase-modified electrodes
    Tanimoto de Albuquerque, Yaico D.
    Franco Ferreira, Lucas
    [J]. ANALYTICA CHIMICA ACTA, 2007, 596 (02) : 210 - 221
  • [6] Immobilisation and bioelectrochemistry of proteins on nanoporous TiO2 and ZnO films
    Topoglidis, E
    Cass, AEG
    O'Regan, B
    Durrant, JR
    [J]. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2001, 517 (1-2): : 20 - 27
  • [7] Factors that affect protein adsorption on nanostructured titania films. A novel spectroelectrochemical application to sensing
    Topoglidis, E
    Campbell, CJ
    Cass, AEG
    Durrant, JR
    [J]. LANGMUIR, 2001, 17 (25) : 7899 - 7906
  • [8] Protein adsorption on nanocrystalline TiO2 films:: An immobilization strategy for bioanalytical devices
    Topoglidis, E
    Cass, AEG
    Gilardi, G
    Sadeghi, S
    Beaumont, N
    Durrant, JR
    [J]. ANALYTICAL CHEMISTRY, 1998, 70 (23) : 5111 - 5113
  • [9] Silica sol-gel composite film as an encapsulation matrix for the construction of an amperometric tyrosinase-based biosensor
    Wang, B
    Zhang, J
    Dong, S
    [J]. BIOSENSORS & BIOELECTRONICS, 2000, 15 (7-8) : 397 - 402
  • [10] Zinc oxide nanocomb biosensor for glucose detection
    Wang, J. X.
    Sun, X. W.
    Wei, A.
    Lei, Y.
    Cai, X. P.
    Li, C. M.
    Dong, Z. L.
    [J]. APPLIED PHYSICS LETTERS, 2006, 88 (23)