Amperometric biosensing of carbamate and organophosphate pesticides utilizing screen-printed tyrosinase-modified electrodes

被引:124
作者
Tanimoto de Albuquerque, Yaico D. [1 ]
Franco Ferreira, Lucas [1 ]
机构
[1] Univ Fed Uberlandia, Inst Quim, Lab Electroquim Aplicada, BR-38400089 Uberlandia, MG, Brazil
关键词
amperometric biosensor; tyrosinase electrode; enzyme inhibitors; pesticides;
D O I
10.1016/j.aca.2007.06.013
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A tyrosinase (Tyr) screen-printed biosensor based on the electroreduction of enzymatically generated quinoid products was electrochemically characterized and optimized for determination of carbamates and organophosphorus pesticides. A composite electrode prepared by screen-printing a cobalt (II) phthalocyanine (CoPc) modified cellulose-graphite composite on a polycarbonate support was employed as electrochemical transducer. The Tyr biosensor was prepared by immobilization of enzyme on the composite electrode surface by cross-linking with glutaraldehyde and bovine serum albumin. Parameters affecting the biosensor response such as response time, enzyme loading, concentration and pH of the buffer solution were optimized utilizing catechol as substrate. The maximum response for o-quinone enzymatically generated was obtained after 2 min of reaction. A good reproducibility and high operational stability were found for Tyr biosensor (60 units) at 50 mM phosphate buffer, pH 6.50. Under these conditions, the useful lifetime of biosensor was 10 days. After 15 days, the biosensor could be used with 20% of the initial value. Inhibition studies on the o-quinone steady-state current (at -0.20 V versus Ag/AgCl) were performed to investigate the inhibition kinetics of the pesticides in the enzymatic activity of mushroom tyrosinase. The results shown that the methyl parathion and carbofuran can lead to competitive inhibition process of the enzyme, while diazinon and carbaryl act as mixed inhibitors. Linear relationships were found for methyl parathion (6-100 ppb), diazinon (19-50 ppb), carbofuran (5-90 ppb) and carbaryl (10-50 ppb). Analysis of natural river water samples spiked with 30 ppb of each pesticide showed recoveries between 92.50% and 98.50% and relative standard deviations of 2%. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:210 / 221
页数:12
相关论文
共 52 条
  • [1] Carbon-polymer biocomposites for amperometric sensing
    Alegret, S
    Cespedes, F
    MartinezFabregas, E
    Martorell, D
    Morales, A
    Centelles, E
    Munoz, J
    [J]. BIOSENSORS & BIOELECTRONICS, 1996, 11 (1-2) : 35 - 44
  • [2] Conductometric tyrosinase biosensor for the detection of diuron, atrazine and its main metabolites
    Anh, TM
    Dzyadevych, SV
    Van, MC
    Renault, NJ
    Duc, CN
    Chovelon, JM
    [J]. TALANTA, 2004, 63 (02) : 365 - 370
  • [3] Solid-phase microextraction in pesticide residue analysis
    Beltran, J
    López, FJ
    Hernández, F
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2000, 885 (1-2) : 389 - 404
  • [4] Structure-based rationalization of urease inhibition by phosphate: novel insights into the enzyme mechanism
    Benini, S
    Rypniewski, WR
    Wilson, KS
    Ciurli, S
    Mangani, S
    [J]. JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 2001, 6 (08): : 778 - 790
  • [5] A BIOSENSOR AS WARNING DEVICE FOR THE DETECTION OF CYANIDE, CHLOROPHENOLS, ATRAZINE AND CARBAMATE PESTICIDES
    BESOMBES, JL
    COSNIER, S
    LABBE, P
    REVERDY, G
    [J]. ANALYTICA CHIMICA ACTA, 1995, 311 (03) : 255 - 263
  • [6] BIOAMPEROMETRIC SENSORS FOR PHENOL BASED ON CARBON PASTE ELECTRODES
    BONAKDAR, M
    VILCHEZ, JL
    MOTTOLA, HA
    [J]. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1989, 266 (01): : 47 - 55
  • [7] Rate-limiting steps of tyrosinase-modified electrodes for the detection of catechol
    Burestedt, E
    Narvaez, A
    Ruzgas, T
    Gorton, L
    Emneus, J
    Dominguez, E
    MarkoVarga, G
    [J]. ANALYTICAL CHEMISTRY, 1996, 68 (09) : 1605 - 1611
  • [8] ANALYSIS OF L-DOPA IN PHARMACEUTICAL PREPARATIONS AND OF TOTAL PHENOLS CONTENT IN URINE BY MEANS OF AN ENZYME AMPEROMETRIC SENSOR
    CAMPANELLA, L
    BEONE, T
    SAMMARTINO, MP
    TOMASSETTI, M
    [J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1993, 11 (11-12) : 1099 - 1104
  • [9] Amperometric flow-injection determination of phenolic compounds at self-assembled monolayer-based tyrosinase biosensors
    Campuzano, S
    Serra, B
    Pedrero, M
    de Villena, FJM
    Pingarrón, JM
    [J]. ANALYTICA CHIMICA ACTA, 2003, 494 (1-2) : 187 - 197
  • [10] Disposable tyrosinase-peroxidase bi-enzyme sensor for amperometric detection of phenols
    Chang, SC
    Rawson, K
    McNeil, CJ
    [J]. BIOSENSORS & BIOELECTRONICS, 2002, 17 (11-12) : 1015 - 1023