Conjugated polyelectrolytes:: Conformation-sensitive optical probes for detection of arnyloid fibril forrnation

被引:149
作者
Nilsson, KPR [1 ]
Herland, A
Hammarström, P
Inganäs, O
机构
[1] Linkoping Univ, Dept Phys & Measurement Technol, SE-58183 Linkoping, Sweden
[2] Linkoping Univ, Dept Biol, SE-58183 Linkoping, Sweden
[3] Linkoping Univ, Dept Chem, SE-58183 Linkoping, Sweden
关键词
D O I
10.1021/bi047402u
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The in vivo deposition of amyloid fibrils is a hallmark of many devastating diseases known n as the amyloidoses. Amyloid formation in vitro may also complicate production of proteins in the biotechnology industry. Simple, sensitive, and versatile tools that detect the fibrillar conformation of amyloidogenic proteins are thus of great importance. We have developed a negatively charged conjugated polyelectrolyte that displays different characteristic optical changes, detected visually or by absorption and emission, depending on whether the protein with which it forms a complex is in its native state or amyloid fibril conformation. This simple, rapid, and novel methodology was applied here to two amyloidogenic proteins, insulin and lysozyme, and its validity for detection of their fibrillar conformation was verified by currently used methods Such as circular dichroism, transmission electron microscopy, and Congo red absorption.
引用
收藏
页码:3718 / 3724
页数:7
相关论文
共 40 条
[1]   Partially folded intermediates in insulin fibrillation [J].
Ahmad, A ;
Millett, IS ;
Doniach, S ;
Uversky, VN ;
Fink, AL .
BIOCHEMISTRY, 2003, 42 (39) :11404-11416
[2]   ATOMIC POSITIONS IN RHOMBOHEDRAL 2-ZINC INSULIN CRYSTALS [J].
BLUNDELL, TL ;
CUTFIELD, JF ;
CUTFIELD, SM ;
DODSON, EJ ;
DODSON, GG ;
HODGKIN, DC ;
MERCOLA, DA ;
VIJAYAN, M .
NATURE, 1971, 231 (5304) :506-&
[3]   Formation of insulin amyloid fibrils followed by FTIR simultaneously with CD and electron microscopy [J].
Bouchard, M ;
Zurdo, J ;
Nettleton, EJ ;
Dobson, CM ;
Robinson, CV .
PROTEIN SCIENCE, 2000, 9 (10) :1960-1967
[4]   Conformational disease [J].
Carrell, RW ;
Lomas, DA .
LANCET, 1997, 350 (9071) :134-138
[5]   Highly sensitive biological and chemical sensors based on reversible fluorescence quenching in a conjugated polymer [J].
Chen, LH ;
McBranch, DW ;
Wang, HL ;
Helgeson, R ;
Wudl, F ;
Whitten, DG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (22) :12287-12292
[6]   Photovoltaic cells with a conjugated polyelectrolyte [J].
Ding, L ;
Jonforsen, M ;
Roman, LS ;
Andersson, MR ;
Inganäs, O .
SYNTHETIC METALS, 2000, 110 (02) :133-140
[7]   Protein misfolding, evolution and disease [J].
Dobson, CM .
TRENDS IN BIOCHEMICAL SCIENCES, 1999, 24 (09) :329-332
[8]   High-efficiency fluorescence quenching of conjugated polymers by proteins [J].
Fan, CH ;
Plaxco, KW ;
Heeger, AJ .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (20) :5642-5643
[9]   DNA hybridization detection with water-soluble conjugated polymers and chromophore-labeled single-stranded DNA [J].
Gaylord, BS ;
Heeger, AJ ;
Bazan, GC .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (04) :896-900
[10]   DNA detection using water-soluble conjugated polymers and peptide nucleic acid probes [J].
Gaylord, BS ;
Heeger, AJ ;
Bazan, GC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (17) :10954-10957