Assessment of the uniformity of wheat and tomato varieties at DNA microsatellite loci

被引：45

作者：

Cooke, RJ

Bredemeijer, GMM

Ganal, MW

Peeters, R

Isaac, P

Rendell, S

Jackson, J

Röder, MS

Korzun, V

Wendehake, K

Areshchenkova, T

Dijcks, M

Laborie, D

Bertrand, L

Vosman, B

机构：

[1] NIAB, Cambridge CB3 0LE, England

[2] Plant Res Int, NL-6700 AA Wageningen, Netherlands

[3] IPK, Inst Plant Genet & Crop Plant Res, D-06466 Gatersleben, Germany

[4] Nunhems Zaden BV, NL-6080 AA Haelen, Netherlands

[5] Agrogene SA, F-77550 Moissy Cramayel, France

来源：

EUPHYTICA | 2003年 / 132卷 / 03期

关键词：

microsatellites; SSR; tomato; uniformity; variety testing; wheat;

D O I：

10.1023/A:1025046919570

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

By analysing a number (20-38) of individuals from selected varieties of wheat and tomato, we have been able to assess intra-varietal uniformity at certain microsatellite (simple sequence repeat, SSR) loci. In total, 45 varieties of wheat were analysed at between 7-9 different SSR loci, and 10 varieties of tomato were analysed at six loci. The results showed that there was variation both between varieties and between microsatellites in the degree of non-uniformity observed, and it was possible to identify a number of different probable sources of non-uniformity. Twenty-four of the wheat varieties and nine of the tomato varieties were sufficiently uniform to meet the standards currently applied for distinctness, uniformity and stability (DUS) testing using phenotypic characteristics. The implications for the potential future use of SSRs in DUS testing are discussed.

引用

页码：331 / 341

页数：11

共 13 条

[1] Construction and testing of a microsatellite database containing more than 500 tomato varieties [J].