Improved axial resolution by point-spread autocorrelation function imaging

被引：4

作者：

Brakenhoff, GJ

Muller, M

机构：

[1] BioCentrum Amsterdam, Department of Molecular Cytology, University of Amsterdam, 1098 SM Amsterdam

来源：

OPTICS LETTERS | 1996年 / 21卷 / 21期

关键词：

D O I：

10.1364/OL.21.001721

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Based on an interferometric spatial autocorrelation of two shifted point-spread functions combined with confocal detection, we demonstrate that the point-spread autocorrelation function technique provides improved axial resolution compared with conventional confocal imaging. The principle of the technique is demonstrated for a one-sided fluorescing step object. (C) 1996 Optical Society of America

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页码：1721 / 1723

页数：3

相关论文

共 13 条

[1] CONFOCAL SCANNING LIGHT-MICROSCOPY WITH HIGH APERTURE IMMERSION LENSES [J].

BRAKENHOFF, GJ ;

BLOM, P ;

BARENDS, P .

JOURNAL OF MICROSCOPY-OXFORD, 1979, 117 (NOV) :219-232

[2] 3-DIMENSIONAL CHROMATIN DISTRIBUTION IN NEURO-BLASTOMA NUCLEI SHOWN BY CONFOCAL SCANNING LASER MICROSCOPY [J].

BRAKENHOFF, GJ ;

VANDERVOORT, HTM ;

VANSPRONSEN, EA ;

LINNEMANS, WAM ;

NANNINGA, N .

NATURE, 1985, 317 (6039) :748-749

[3] FUNDAMENTAL IMPROVEMENT OF RESOLUTION WITH A 4PI-CONFOCAL FLUORESCENCE MICROSCOPE USING 2-PHOTON EXCITATION [J].

HELL, S ;

STELZER, EHK .

OPTICS COMMUNICATIONS, 1992, 93 (5-6) :277-282

[4] ABERRATIONS IN CONFOCAL FLUORESCENCE MICROSCOPY INDUCED BY MISMATCHES IN REFRACTIVE-INDEX [J].

HELL, S ;

REINER, G ;

CREMER, C ;

STELZER, EHK .

JOURNAL OF MICROSCOPY, 1993, 169 :391-405

[5] MEASUREMENT OF THE 4PI-CONFOCAL POINT-SPREAD FUNCTION PROVES 75 NM AXIAL RESOLUTION [J].

HELL, SW ;

LINDEK, S ;

CREMER, C ;

STELZER, EHK .

APPLIED PHYSICS LETTERS, 1994, 64 (11) :1335-1337

[6] BREAKING THE DIFFRACTION RESOLUTION LIMIT BY STIMULATED-EMISSION - STIMULATED-EMISSION-DEPLETION FLUORESCENCE MICROSCOPY [J].

HELL, SW ;

WICHMANN, J .

OPTICS LETTERS, 1994, 19 (11) :780-782

[7] IMPROVEMENT OF LATERAL RESOLUTION IN FAR-FIELD FLUORESCENCE LIGHT-MICROSCOPY BY USING 2-PHOTON EXCITATION WITH OFFSET BEAMS [J].

HELL, SW .

OPTICS COMMUNICATIONS, 1994, 106 (1-3) :19-24

[8] CHARACTERIZATION OF HIGH-NUMERICAL-APERTURE LENSES BY SPATIAL AUTOCORRELATION OF THE FOCAL FIELD [J].

MULLER, M ;

BRAKENHOFF, GJ .

OPTICS LETTERS, 1995, 20 (21) :2159-2161

[9] IMAGE-FORMATION IN SCANNING MICROSCOPE [J].

SHEPPARD, CJR ;

CHOUDHURY, A .

OPTICA ACTA, 1977, 24 (10) :1051-1073

[10] FUNDAMENTAL REDUCTION OF THE OBSERVATION VOLUME IN FAR-FIELD LIGHT-MICROSCOPY BY DETECTION ORTHOGONAL TO THE ILLUMINATION AXIS - CONFOCAL THETA MICROSCOPY [J].

STELZER, EHK ;

LINDEK, S .

OPTICS COMMUNICATIONS, 1994, 111 (5-6) :536-547