Direct electrochemistry of cytochrome c on a multi-walled carbon nanotubes modified electrode and its electrocatalytic activity for the reduction of H2O2

被引:212
作者
Zhao, GC [1 ]
Yin, ZZ [1 ]
Zhang, L [1 ]
Wei, XW [1 ]
机构
[1] Anhui Normal Univ, Sch Chem & Mat Sci, Anhui Key Lab Funct Mol Solids, Wuhu 241000, Peoples R China
基金
中国国家自然科学基金;
关键词
cytochrome c; multi-walled carbon nanotubes; direct electrochemistry; biosensor;
D O I
10.1016/j.elecom.2005.01.006
中图分类号
O646 [电化学、电解、磁化学];
学科分类号
081704 ;
摘要
Direct electrochemistry of cytochrome c (Cyt c), which was adsorbed on the surface of multi-walled carbon nanotubes (MWNTs), was investigated. The results from electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) suggested that Cyt c could be tightly adsorbed on the surface of MWNTs and MWNTs show an obvious promotion for the direct electro-transfer between Cyt c and electrode. A couple of well-defined and quasi-reversible CV peaks of Cyt c can be observed in a phosphate buffer solution (pH 7.0). Cyt c adsorbed on MWNTs exhibits a remarkable electrocatalytic activity for the reduction of hydrogen peroxide (11201). The calculated apparent Michaelis-Menten constant (K-m(app)) was 857 muM, indicating a high catalytic activity of Cyt c adsorbed on MWNTs modified electrode to the reduction of H2O2. Based on these, a third generation reagentless biosensor can be constructed for the determination of H2O2. (C) 2005 Elsevier B.V. All rights reserved.
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页码:256 / 260
页数:5
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