Higher-energy C-trap dissociation for peptide modification analysis

被引：715

作者：

Olsen, Jesper V.

Macek, Boris

Lange, Oliver

Makarov, Alexander

Horning, Stevan

Mann, Matthias

机构：

[1] Thermo Fisher Sci GmbH, D-28199 Bremen, Germany

[2] Max Planck Inst Biochem, Dept Proteom & Signal Transduc, D-82131 Martinsried, Germany

来源：

NATURE METHODS | 2007年 / 4卷 / 09期

关键词：

D O I：

10.1038/NMETH1060

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Peptide sequencing is the basis of mass spectrometry-driven proteomics. Here we show that in the linear ion trap-orbitrap mass spectrometer (LTQ Orbitrap) peptide ions can be efficiently fragmented by high- accuracy and full-mass-range tandem mass spectrometry (MS/MS) via higher-energy C-trap dissociation (HCD). Immonium ions generated via HCD pinpoint modifications such as phosphotyrosine with very high confidence. Additionally we show that an added octopole collision cell facilitates de novo sequencing.

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收藏

页码：709 / 712

页数：4

相关论文

共 16 条

[1] Mass spectrometry-based proteomics [J].

Aebersold, R ;

Mann, M .

NATURE, 2003, 422 (6928) :198-207

[2] Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics [J].

Blagoev, B ;

Ong, SE ;

Kratchmarova, I ;

Mann, M .

NATURE BIOTECHNOLOGY, 2004, 22 (09) :1139-1145

[3] Mapping posttranslational modifications of proteins by MS-based selective detection: Application to phosphoproteomics [J].

Carr, SA ;

Annan, RS ;

Huddleston, MJ .

MASS SPECTROMETRY: MODIFIED PROTEINS AND GLYCOCONJUGATES, 2005, 405 :82-+

[4] The Orbitrap: a new mass spectrometer [J].

Hu, QZ ;

Noll, RJ ;

Li, HY ;

Makarov, A ;

Hardman, M ;

Cooks, RG .

JOURNAL OF MASS SPECTROMETRY, 2005, 40 (04) :430-443

[5] Probing lysine acetylation with a modification-specific marker ion using high-performance liquid chromatography/electrospray-mass spectrometry with collision-induced dissociation [J].

Kim, JY ;

Kim, KW ;

Kwon, HJ ;

Lee, DW ;

Yoo, JS .

ANALYTICAL CHEMISTRY, 2002, 74 (21) :5443-5449

[6] INSTRUMENTATION, APPLICATIONS, AND ENERGY DEPOSITION IN QUADRUPOLE ION-TRAP TANDEM MASS-SPECTROMETRY [J].

LOURIS, JN ;

COOKS, RG ;

SYKA, JEP ;

KELLEY, PE ;

STAFFORD, GC ;

TODD, JFJ .

ANALYTICAL CHEMISTRY, 1987, 59 (13) :1677-1685

[7] Performance evaluation of a hybrid linear ion trap/orbitrap mass spectrometer [J].

Makarov, A ;

Denisov, E ;

Kholomeev, A ;

Baischun, W ;

Lange, O ;

Strupat, K ;

Horning, S .

ANALYTICAL CHEMISTRY, 2006, 78 (07) :2113-2120

[8] ERROR TOLERANT IDENTIFICATION OF PEPTIDES IN SEQUENCE DATABASES BY PEPTIDE SEQUENCE TAGS [J].

MANN, M ;

WILM, M .

ANALYTICAL CHEMISTRY, 1994, 66 (24) :4390-4399

[9] Parts per million mass accuracy on an orbitrap mass spectrometer via lock mass injection into a C-trap [J].

Olsen, JV ;

de Godoy, LMF ;

Li, GQ ;

Macek, B ;

Mortensen, P ;

Pesch, R ;

Makarov, A ;

Lange, O ;

Horning, S ;

Mann, M .

MOLECULAR & CELLULAR PROTEOMICS, 2005, 4 (12) :2010-2021

[10] Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics [J].

Ong, SE ;

Blagoev, B ;

Kratchmarova, I ;

Kristensen, DB ;

Steen, H ;

Pandey, A ;

Mann, M .

MOLECULAR & CELLULAR PROTEOMICS, 2002, 1 (05) :376-386