Detection of microRNAs by Northern blot analyses using LNA probes

被引：96

作者：

Varallyay, Eva ^{[1
]}

Burgyan, Jozsef ^{[1
]}

Havelda, Zoltan ^{[1
]}

机构：

[1] Agr Biotechnol Ctr, H-2100 Godollo, Hungary

来源：

METHODS | 2007年 / 43卷 / 02期

基金：

匈牙利科学研究基金会;

关键词：

MicroRNA; LNA (locked nucleic acid); Northern blot;

D O I：

10.1016/j.ymeth.2007.04.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

MicroRNAs (miRNAs) are small, endogenous, non-coding RNA species, about 21 nucleotides in length, which modulate the expression of animal and plant target genes at the post-transcriptional level. It has been shown that miRNA based gene regulation plays a pivotal role in pathways involved in growth and development. Understanding miRNA mediated processes requires new technologies enabling efficient detection of small RNA species. Here we report the optimization of a miRNA Northern blot detection method based on LNA (locked nucleic acid)-modified oligonucleotide hybridization. This technique allows sensitive and highly specific detection of mature miRNAs. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：140 / 145

页数：6

共 8 条

[1] Locked nucleic acid (LNA): fine-tuning the recognition of DNA and RNA [J].