Electrospray micromixer chip for on-line derivatization and kinetic studies

被引:22
作者
Abonnenc, Melanie [1 ]
Dayon, Lorc [1 ]
Perruche, Brice [1 ]
Lion, Niels [1 ]
Girault, Hubert H. [1 ]
机构
[1] Ecole Polytech Fed Lausanne, SB, ISIC, LEPA,Stn 6, CH-1015 Lausanne, Switzerland
关键词
D O I
10.1021/ac800058h
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An electrospray microchip for mass spectrometry comprising an integrated passive mixer to carry out on-chip chemical derivatizations is described. The microchip fabricated using UV-photoablation is composed of two microchannels linked together by a liquid junction. Downstream of this liquid junction, a mixing unit made of parallel oblique grooves is integrated to the microchannel in order to create flow perturbations. Several mixer designs are evaluated. The mixer efficiency is investigated both by fluorescence study and mass spectrometric monitoring of the tagging reaction of cysteinyl peptides with 1,4-benzoquinone. The comparisons with a microchip without a mixing unit and a kinetic model are used to assess the efficiency of the mixer showing tagging kinetics close to that of bulk reactions in an ideally mixed reactor. As an ultimate application, the electrospray micromixer is implemented in a LC-MS workflow. Online derivatization of albumin tryptic peptides after a reversed-phase separation and counting of their cysteines drastically enhance the protein identification.
引用
收藏
页码:3372 / 3378
页数:7
相关论文
共 36 条
[1]   Mass spectrometry-based proteomics [J].
Aebersold, R ;
Mann, M .
NATURE, 2003, 422 (6928) :198-207
[2]   Miniaturized continuous flow reaction vessels: influence on chemical reactions [J].
Brivio, M ;
Verboom, W ;
Reinhoudt, DN .
LAB ON A CHIP, 2006, 6 (03) :329-344
[3]   Chip-based on-line nanospray MS method enabling study of the kinetics of isocyanate derivatization reactions [J].
Brivio, M ;
Liesener, A ;
Osterbroek, RE ;
Verboom, W ;
Karst, U ;
van den Berg, A ;
Reinhoudt, DN .
ANALYTICAL CHEMISTRY, 2005, 77 (21) :6852-6856
[4]   Probing cysteine reactivity in proteins by mass spectrometric EC-tagging [J].
Dayon, L ;
Roussel, C ;
Girault, HH .
JOURNAL OF PROTEOME RESEARCH, 2006, 5 (04) :793-800
[5]   On-line counting of cysteine residues in peptides during electrospray ionization by electrogenerated tags and their application to protein identification [J].
Dayon, L ;
Roussel, C ;
Prudent, M ;
Lion, N ;
Girault, HH .
ELECTROPHORESIS, 2005, 26 (01) :238-247
[6]   Diagonal chromatographic selection of cysteinyl peptides modified with benzoquinones [J].
Dayon, Loic ;
Girault, Hubert H. .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2007, 389 (03) :841-849
[7]   Multitrack electrospray chips [J].
Dayon, Loic ;
Abonnenc, Melanie ;
Prudent, Michel ;
Lion, Niels ;
Girault, Hubert H. .
JOURNAL OF MASS SPECTROMETRY, 2006, 41 (11) :1484-1490
[8]   Demonstration of direct bioanalysis of drugs in plasma using nanoelectrospray infusion from a silicon chip coupled with tandem mass spectrometry [J].
Dethy, JM ;
Ackermann, BL ;
Delatour, C ;
Henion, JD ;
Schultz, GA .
ANALYTICAL CHEMISTRY, 2003, 75 (04) :805-811
[9]   An example of a chaotic micromixer: the cross-channel micromixer [J].
Dodge, A ;
Jullien, MC ;
Lee, YK ;
Niu, X ;
Okkels, F ;
Tabeling, P .
COMPTES RENDUS PHYSIQUE, 2004, 5 (05) :557-563
[10]   Review - Mass spectrometry and protein analysis [J].
Domon, B ;
Aebersold, R .
SCIENCE, 2006, 312 (5771) :212-217