Activity and stability of native and modified subtilisins in various media

被引：12

作者：

Bacheva, AV

Baibak, OV

Belyaeva, AV

Oksenoit, ES

Velichko, TI

Lysogorskaya, EN

Gladilin, AK

Lozinsky, VI

Filippova, IY ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Fac Chem, Moscow 119992, Russia

[2] Russian Acad Sci, AN Nesmeyanov Organoelement Cpds Inst, Moscow 119991, Russia

来源：

BIOCHEMISTRY-MOSCOW | 2003年 / 68卷 / 11期

基金：

俄罗斯基础研究基金会;

关键词：

immobilized subtilisin; poly(vinyl alcohol) cryogel; complex of subtilisin with poly(acrylic acid); organic solvents;

D O I：

10.1023/B:BIRY.0000009142.88974.85

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The activity and stability of native subtilisin 72, its complex with poly(acrylic acid), and subtilisin covalently attached to poly(vinyl alcohol) cryogel were studied in aqueous and organic media by hydrolysis of specific chromogenic peptide substrates. Kinetic parameters of the hydrolysis of Glp-Ala-Ala-Leu-pNA by native subtilisin and its complex with poly(acrylic acid) were determined. Based on the comparative study of stability of native and modified subtilisins in media of various compositions, it was established that covalent immobilization of subtilisin on poly(vinyl alcohol) cryogel is the most effective approach to improve enzyme stability in water as well as in mixtures with low water content.

引用

页码：1261 / 1266

页数：6

共 27 条

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