Process of folding proteins into membranes: Challenges and progress

被引：76

作者：

Stanley, Ann Marie ^{[1
]}

Fleming, Karen G. ^{[1
]}

机构：

[1] Johns Hopkins Univ, TC Jenkins Dept Biophys, Baltimore, MD 21218 USA

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2008年 / 469卷 / 01期

基金：

美国国家科学基金会;

关键词：

membrane protein; thermodynamics; protein folding;

D O I：

10.1016/j.abb.2007.09.024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Just 25 years ago the Anfinsen thermodynamic hypothesis was shown to be valid for membrane proteins. Despite the complex biological machinery required for their in vivo assembly and in face of the chemically heterogeneous, anisotropic nature of their biological lipid bilaver "solvent", the evidence continues to suggest that membrane proteins are equilibrium structures. The progress in finding conditions in vitro to investigate the physical origins of their stabilities is the focus of this article. We catalogue in vitro folding studies in detergent micelles and in lipid bilayers. We consider the unique technical obstacles to folding studies of membrane proteins, and we highlight the progress that has been made in quantitative descriptions of membrane protein stability. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：46 / 66

页数：21

共 125 条

[1] STRUCTURE OF THE REACTION CENTER FROM RHODOBACTER-SPHAEROIDES R-26 - THE COFACTORS .1. [J].

ALLEN, JP ;

FEHER, G ;

YEATES, TO ;

KOMIYA, H ;

REES, DC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (16) :5730-5734

[2] Controlling the folding efficiency of an integral membrane protein [J].

Allen, SJ ;

Curran, AR ;

Templer, RH ;

Meijberg, W ;

Booth, PJ .

JOURNAL OF MOLECULAR BIOLOGY, 2004, 342 (04) :1293-1304

[3] Folding kinetics of an α helical membrane protein in phospholipid bilayer vesicles [J].

Allen, SJ ;

Curran, AR ;

Templer, RH ;

Meijberg, W ;

Booth, PJ .

JOURNAL OF MOLECULAR BIOLOGY, 2004, 342 (04) :1279-1291

[4] Structure and function in bacteriorhodopsin: The effect of the interhelical loops on the protein folding kinetics [J].

Allen, SJ ;

Kim, JM ;

Khorana, HG ;

Lu, H ;

Booth, PJ .

JOURNAL OF MOLECULAR BIOLOGY, 2001, 308 (02) :423-435

[5] PRINCIPLES THAT GOVERN FOLDING OF PROTEIN CHAINS [J].

ANFINSEN, CB .

SCIENCE, 1973, 181 (4096) :223-230

[6] Unfolding and refolding in vitro of a tetrameric, α-helical membrane protein:: The prokaryotic potassium channel KcsA [J].

Barrera, FN ;

Renart, ML ;

Molina, ML ;

Poveda, JA ;

Encinar, JA ;

Fernández, AM ;

Neira, JL ;

González-Ros, JM .

BIOCHEMISTRY, 2005, 44 (43) :14344-14352

[7] Irreversible assembly of membrane fusion machines [J].

Barrick, D ;

Hughson, FM .

NATURE STRUCTURAL BIOLOGY, 2002, 9 (02) :78-80

[8] Projection structure of the monomeric porin OmpG at 6 Å resolution [J].

Behlau, M ;

Mills, DJ ;

Quader, H ;

Kühlbrandt, W ;

Vonck, J .

JOURNAL OF MOLECULAR BIOLOGY, 2001, 305 (01) :71-77

[9] Assembly of light-harvesting chlorophyll a/b complex in vitro. Time-resolved fluorescence measurements [J].

Booth, PJ ;

Paulsen, H .

BIOCHEMISTRY, 1996, 35 (16) :5103-5108

[10] INTERMEDIATES IN THE FOLDING OF THE MEMBRANE-PROTEIN BACTERIORHODOPSIN [J].

BOOTH, PJ ;

FLITSCH, SL ;

STERN, LJ ;

GREENHALGH, DA ;

KIM, PS ;

KHORANA, HG .

NATURE STRUCTURAL BIOLOGY, 1995, 2 (02) :139-143

← 1 2 3 4 5 6 7 8 9 10 →