Straightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometry

被引：26

作者：

da Silva, Ricardo M. P. ^{[1
,2
]}

Mano, Jado F. ^{[1
,2
]}

Reis, Rui L. ^{[1
,2
]}

机构：

[1] Univ Minho, Dept Polymer Engn, Res Grp 3Bs, P-4710057 Braga, Portugal

[2] IBB, PT Govt Associated Lab, Braga, Portugal

来源：

MACROMOLECULAR CHEMISTRY AND PHYSICS | 2008年 / 209卷 / 14期

关键词：

acetylation; chitin; chitosan; deacetylation; NMR; UV spectrophotometry;

D O I：

10.1002/macp.200800191

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 [高分子化学与物理]; 080501 [材料物理与化学]; 081704 [应用化学];

摘要：

First-derivative UV spectrophotometry is shown to be a reliable method for the determination of the degree of N-acetylation of chitosan samples. A mathematical expression is derived that allows to determine the DA directly from the mass concentration of a chitosan solution and the first derivative of its UV spectrum at 202 nm, thus eliminating the need for empiric correction curves for highly deacetylated samples. A procedure is proposed for the accurate mass determination of the hygroscopic chitosan. The proposed approach facilitates the routine determination of the DA, especially when using potent multiwell microplate readers, which allow hundreds of samples to be measured in just a few minutes.

引用

页码：1463 / 1472

页数：10

共 33 条

[1]

Multichannel mould processing of 3D structures from microporous coralline hydroxyapatite granules and chitosan support materials for guided tissue regeneration/engineering [J].