Multilayer poly(vinyl alcohol)-adsorbed coating on poly(dimethylsiloxane) microfluidic chips for biopolymer separation

被引：126

作者：

Wu, DP ^{[1
]}

Luo, Y ^{[1
]}

Zhou, XM ^{[1
]}

Dai, ZP ^{[1
]}

Lin, BC ^{[1
]}

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

来源：

ELECTROPHORESIS | 2005年 / 26卷 / 01期

关键词：

microfluidic chip; miniaturization; poly(dimethylsiloxane); poly(vinyl alcohol);

D O I：

10.1002/elps.200406157

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A poly(dimethylsiloxane) (PDMS) microfluidic chip surface was modified by multilayer-adsorbed and heat-immobilized poly(vinyl alcohol) (PVA) after oxygen plasma treatment. The reflection absorption infrared spectrum (RAIRS) showed that 88% hydrolyzed PVA adsorbed more strongly than 100% hydrolyzed one on the oxygen plasma-pretreated PDMS surface, and they all had little adsorption on original PDMS surface. Repeating the coating procedure three times was found to produce the most robust and effective coating. PVA coating converted the original PDMS surface from a hydrophobic one into a hydrophilic surface, and suppressed electroosmotic flow (EOF) in the range of pH 3-11. More than 1 000 000 plates/m and baseline resolution were obtained for separation of fluorescently labeled basic proteins (lysozyme, ribonuclease B). Fluorescently labeled acidic proteins (bovine serum albumin, P-lactoglobulin) and fragments of dsDNA phiX174 RF/HaeIII were also separated satisfactorily in the three-layer 88% PVA-coated PDMS microchip. Good separation of basic proteins was obtained for about 70 consecutive runs.

引用

页码：211 / 218

页数：8

共 43 条

[21] Reactive polymer coatings: A first step toward surface engineering of microfluidic devices [J].

Lahann, J ;

Balcells, M ;

Lu, H ;

Rodon, T ;

Jensen, KF ;

Langer, R .

ANALYTICAL CHEMISTRY, 2003, 75 (09) :2117-2122

[22] Surface biopassivation of replicated poly(dimethylsiloxane) microfluidic channels and application to heterogeneous immunoreaction with on-chip fluorescence detection [J].

Linder, V ;

Verpoorte, E ;

Thormann, W ;

de Rooij, NF ;

Sigrist, M .

ANALYTICAL CHEMISTRY, 2001, 73 (17) :4181-4189

[23] Dynamic coating using polyelectrolyte multilayers for chemical control of electroosmotic flow in capillary electrophoresis microchips [J].

Liu, Y ;

Fanguy, JC ;

Bledsoe, JM ;

Henry, CS .

ANALYTICAL CHEMISTRY, 2000, 72 (24) :5939-5944

[24] Coated microfluidic devices for improved chiral separations in microchip electrophoresis [J].

Ludwig, M ;

Belder, D .

ELECTROPHORESIS, 2003, 24 (15) :2481-2486

[25] Surface modification of poly(dimethylsiloxane) microchannels [J].

Makamba, H ;

Kim, JH ;

Lim, K ;

Park, N ;

Hahn, JH .

ELECTROPHORESIS, 2003, 24 (21) :3607-3619

[26] MINIATURIZED TOTAL CHEMICAL-ANALYSIS SYSTEMS - A NOVEL CONCEPT FOR CHEMICAL SENSING [J].

MANZ, A ;

GRABER, N ;

WIDMER, HM .

SENSORS AND ACTUATORS B-CHEMICAL, 1990, 1 (1-6) :244-248

[27] Prototyping of microfluidic devices in poly(dimethylsiloxane) using solid-object printing [J].

McDonald, JC ;

Chabinyc, ML ;

Metallo, SJ ;

Anderson, JR ;

Stroock, AD ;

Whitesides, GM .

ANALYTICAL CHEMISTRY, 2002, 74 (07) :1537-1545

[28]

McDonald JC, 2000, ELECTROPHORESIS, V21, P27, DOI 10.1002/(SICI)1522-2683(20000101)21:1<27::AID-ELPS27>3.0.CO

[29]

2-C

[30] DNA capillary electrophoresis using poly(vinyl alcohol). I. Inner capillary coating [J].

Moritani, T ;

Yoon, K ;

Rafailovich, M ;

Chu, B .

ELECTROPHORESIS, 2003, 24 (16) :2764-2771

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