MicroRNA profiling: approaches and considerations

被引:1292
作者
Pritchard, Colin C. [2 ]
Cheng, Heather H. [3 ]
Tewari, Muneesh [1 ,3 ,4 ]
机构
[1] Fred Hutchinson Canc Res Ctr, Human Biol Div, Seattle, WA 98104 USA
[2] Univ Washington, Dept Lab Med, Seattle, WA 98195 USA
[3] Univ Washington, Dept Med, Seattle, WA USA
[4] Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, Seattle, WA 98104 USA
基金
美国国家卫生研究院;
关键词
DIGITAL GENE-EXPRESSION; REAL-TIME PCR; SMALL RNAS; MESSENGER-RNA; DIFFERENTIAL EXPRESSION; CIRCULATING MICRORNAS; QUANTITATIVE PCR; 3' ADENYLATION; MIRNA; QUANTIFICATION;
D O I
10.1038/nrg3198
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
MicroRNAs (miRNAs) are small RNAs that post-transcriptionally regulate the expression of thousands of genes in a broad range of organisms in both normal physiological contexts and in disease contexts. miRNA expression profiling is gaining popularity because miRNAs, as key regulators in gene expression networks, can influence many biological processes and also show promise as biomarkers for disease. Technological advances have spawned a multitude of platforms for miRNA profiling, and an understanding of the strengths and pitfalls of different approaches can aid in their effective use. Here, we review the major considerations for carrying out and interpreting results of miRNA-profiling studies.
引用
收藏
页码:358 / 369
页数:12
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