Gene discovery by ribozyme and siRNA libraries

被引：34

作者：

Akashi, H

Matsumoto, S

Taira, K

机构：

[1] Natl Inst Adv Ind Sci & Technol, Gene Funct Res Ctr, Tsukuba, Ibaraki 3058562, Japan

[2] Univ Tokyo, Dept Internal Med, Grad Sch Med, Tokyo 1138655, Japan

[3] Univ Tokyo, Sch Engn, Dept Chem & Biotechnol, Tokyo 1138656, Japan

来源：

NATURE REVIEWS MOLECULAR CELL BIOLOGY | 2005年 / 6卷 / 05期

关键词：

D O I：

10.1038/nrm1646

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Catalytic RNAs, also known as ribozymes, can be engineered to optimize their activities in the intracellular environment. The introduction of a library of active ribozymes into cells, and the subsequent screening for phenotypic changes, allows the rapid identification of gene function. For the determination of gene function, ribozyme technology complements another RNA-based tool that is based on libraries of small interfering RNAs.

引用

页码：413 / 422

页数：10

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