Recombinant thermophilic enzymes for trehalose and trehalosyl dextrins production

被引：17

作者：

de Pascale, D

Sasso, MP

Di Lernia, I

Di Lazzaro, A

Furia, A

Farina, MC

Rossi, M

De Rosa, M

机构：

[1] Univ Naples 2, CRISCEB, Fac Med, Ist Farmacol & Tossicol, I-80134 Naples, Italy

[2] Univ Naples, Dipartimento Chim Organ & Biol, I-80134 Naples, Italy

[3] CNR, CRIB, Ist Biochim Prot & Enzimol, I-80127 Naples, Italy

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2001年 / 11卷 / 4-6期

关键词：

extremophiles; starch; trehalose; trehalose producing enzymes; E; coli;

D O I：

10.1016/S1381-1177(00)00053-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two thermophilic and thermostable enzymes, trehalosyl dextrins forming enzyme (TDFE) and trehalose forming enzyme (TFE), able to convert starch and dextrins to alpha,alpha -trehalose were recently purified and characterized from Sulfolobales [I. Di Lernia, A. Morana, A. Ottombrino, S. Fusco, M. Rossi, M. De Rosa, Extremophiles, 2 (1998) 409; T. Nakada, S. Ikegami, H. Chaen, M. Kubota, S. Fukuda, T. Sugimoto, M. Kurimoto, Y. Tsujisaka, Biosci., Biotechnol., Biochem., 60 (1996) 267; T. Nakada, S. Ikegami, H. Chaen, M. Kubota, S. Fukuda, T. Sugimoto, M. Kurimoto, Y. Tsujisaka, Biosci., Biotechnol., Biochem., 60 (1996) 263; M. Kato, Y. Miura, M. Kettoku, K. Shindo, A. Iwamatsu, K. Kobayashi, Biosci., Biotechnol., Biochem., 60 (1996) 921; M. Kato, Y. Miura, M. Kettoku, K. Shindo, A. Iwamatsu, K. Kobayashi, Biosci., Biotechnol., Biochem., 60 (1996) 925]. The first enzyme transforms starch and dextrins to the corresponding trehalosyl derivatives, with an intramolecular transglycosylation process, which converts the glucosidic linkage at the reducing end from alpha -1,4 to alpha -1,1. The second, hydrolyzes the alpha -1,4 Linkage adjacent to the alpha -1,1 bond of trehalosyl dextrins, forming trehalose and lower molecular weight dextrins. Herein, we report the cloning and high level expression of the two enzymes of Sulfolobus solfataricus;strain MT4 in Escherichia coli using pTrc expression vector. The yield of TDFE and TFE obtained in this expression system was of 180 U/1 and of 3630 U/1 of medium, respectively. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：777 / 786

页数：10

共 10 条

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