Human Alu RNA is a modular transacting repressor of mRNA transcription during heat shock

被引:388
作者
Mariner, Peter D. [1 ]
Walters, Ryan D. [1 ]
Espinoza, Celso A. [1 ]
Drullinger, Linda F. [1 ]
Wagner, Stacey D. [1 ]
Kugel, Jennifer F. [1 ]
Goodrich, James A. [1 ]
机构
[1] Univ Colorado, Dept Chem & Biochem, Boulder, CO 80309 USA
关键词
D O I
10.1016/j.molcel.2007.12.013
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Noncoding RNAs (ncRNAs) have recently been discovered to regulate mRNA transcription in trans, a role traditionally reserved for proteins. The breadth of ncRNAs as transacting transcriptional regulators and the diversity of signals to which they respond are only now becoming recognized. Here we show that human Alu RNA, transcribed from short interspersed elements (SINEs), is a transacting transcriptional repressor during the cellular heat shock response. Alu RNA blocks transcription by binding RNA polymerase II (Pol II) and entering complexes at promoters in vitro and in human cells. Transcriptional repression by Alu RNA involves two loosely structured domains that are modular, a property reminiscent of classical protein transcriptional regulators. Two other SINE RNAs, human scAlu RNA and mouse 131 RNA, also bind Pol 11 but do not repress transcription in vitro. These studies provide an explanation for why mouse cells harbor two major classes of SINEs, whereas human cells contain only one.
引用
收藏
页码:499 / 509
页数:11
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