Enhanced leukocyte-platelet cross-talk in Type 1 diabetes mellitus:: relationship to microangiopathy

被引:53
作者
Hu, H
Li, N
Yngen, M
Östenson, CG
Wallén, NH
Hjemdahl, P [1 ]
机构
[1] Karolinska Hosp, Dept Med, Div Clin Pharmacol, SE-17176 Stockholm, Sweden
[2] Karolinska Hosp, Dept Mol Med, Endocrinol & Diabet Unit, SE-17176 Stockholm, Sweden
[3] Danderyd Hosp, Dept Internal Med, Stockholm, Sweden
关键词
leukocytes; microangiopathy; platelet-leukocyte cross-talk; platelets; Type I diabetes mellitus;
D O I
10.1111/j.1538-7836.2003.00525.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background: Platelets and leukocytes may influence each others' function, i.e. platelet-leukocyte cross-talk. Diabetes mellitus (DM) is associated with platelet and leukocyte dysfunction. Objective: To evaluate platelet-leukocyte cross-talk, and if this might contribute to platelet and leukocyte dysfunction and microangiopathy in DM patients. Patients and methods: We evaluated platelet and leukocyte function, and cross-talk between these cells in Type 1 DM patients without (n=19) and with (n=20) microangiopathy, and healthy subjects (n=27), using whole blood flow cytometry. Platelet-leukocyte cross-talk was studied in hirudinized whole blood incubated at 37degreesC with stirring. Results: Basal single platelet P-selectin and leukocyte CD11b expression were similar in DM patients and healthy subjects, whilst circulating platelet-leukocyte aggregates and plasma elastase levels were elevated in DM patients. The thromboxane A(2) analog U46619 (3x10(-7) M) induced more marked increases of platelet P-selectin expression and platelet-leukocyte aggregation in DM patients than in healthy subjects. The leukocyte-specific agonist N-formylmethionyl-leucyl-phenylalanine (fMLP) (10(-7) M) induced more marked CD11b expression in DM patients with microangiopathy, compared with healthy subjects. Platelet-leukocyte cross-talk induced by U46619 (10(-6) M) showed no difference between DM patients and healthy subjects. fMLP (10(-6) M) evoked marked leukocyte activation, which subsequently caused mild platelet P-selectin expression. This leukocyte-platelet cross-talk was more pronounced in DM patients than in healthy subjects. Furthermore, enhanced leukocyte-platelet cross-talk was correlated to platelet hyperreactivity among DM patients with microangiopathy only. Conclusions: Type 1 DM is associated with platelet and leukocyte hyperactivity, and enhanced leukocyte-platelet cross-talk, which may contribute to platelet hyperactivity and the microvascular complications seen in Type 1 DM.
引用
收藏
页码:58 / 64
页数:7
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