Polypyrrole electrospotting for the construction of oligonucleotide arrays compatible with a surface plasmon resonance hybridization detection.

被引：34

作者：

Livache, T ^{[1
]}

Guedon, P

Brakha, C

Roget, A

Levy, Y

Bidan, G

机构：

[1] Univ J Fourier, Electrochim Mol Lab, UMR 5819,Dept Rech Fondamentale Mat Condensee, CEA,Serv Interfaces & Mat Mol & Macromol, F-38054 Grenoble 9, France

[2] Ctr Sci Orsay, Inst Opt Theor & Applicat, F-91403 Orsay, France

来源：

SYNTHETIC METALS | 2001年 / 121卷 / 1-3期

关键词：

electrospotting; DNA hybridization; polypyrrole; surface plasmon resonance; fluorescence;

D O I：

10.1016/S0379-6779(00)01238-8

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

We describe in this paper a new electrospotting process allowing a straightforward functionalization of homogeneous gold surfaces. It is based on a direct electro-copolymerization of pyrrole and pyrrole linked to an oligonucleotide on a gold substrate via the use of a moving micro electrochemical cell. The polypyrrole spots obtained by this way were characterized in terms of thickness and DNA hybridization efficiency. Results show that either fluorescence or surface plasmon resonance detection of DNA hybridization can be carried out on these substrates.

引用

页码：1443 / 1444

页数：2

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