A DNA-gold nanoparticle-based colorimetric competition assay for the detection of cysteine

被引:454
作者
Lee, Jae-Seung [1 ]
Ulmann, Pirmin A. [1 ]
Han, Min Su [1 ]
Mirkin, Chad A. [1 ]
机构
[1] Northwestern Univ, Int Inst Nanotechnol, Dept Chem, Evanston, IL 60208 USA
关键词
D O I
10.1021/nl0727563
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We report the development of a highly sensitive and selective colorimetric detection method for cysteine based upon oligonucleotide-functionalized gold nanoparticle probes that contain strategically placed thymidine-thymidine (T-T) mismatches complexed with Hg2+. This assay relies upon the distance-dependent optical properties of gold nanoparticles, the sharp melting transition of oligonucleotide-linked nanoparticle aggregates, and the very selective coordination of Hg2+ with cysteine. The concentration of cysteine can be determined by monitoring with the naked eye or a UV-vis spectrometer the temperature at which the purple-to-red color change associated with aggregate dissociation takes place. This assay does not utilize organic cosolvents, enzymatic reactions, light-sensitive dye molecules, lengthy protocols, or sophisticated instrumentation thereby overcoming some of the limitations of more conventional methods.
引用
收藏
页码:529 / 533
页数:5
相关论文
共 67 条
[1]   Organization of 'nanocrystal molecules' using DNA [J].
Alivisatos, AP ;
Johnsson, KP ;
Peng, XG ;
Wilson, TE ;
Loweth, CJ ;
Bruchez, MP ;
Schultz, PG .
NATURE, 1996, 382 (6592) :609-611
[2]   TRANS-2-STYRYLBENZOTHIAZOLE COMPLEXES WITH MERCURY(II) HALIDES - SYNTHESIS, CHARACTERIZATION AND X-RAY CRYSTAL-STRUCTURE OF DIIODOBIS(TRANS-2-STYRYLBENZOTHIAZOLE)MERCURY(II) [J].
BATTAGLIA, LP ;
CORRADI, AB ;
CRAMAROSSA, MR ;
VEZZOSI, IM ;
GIUSTI, JG .
POLYHEDRON, 1993, 12 (18) :2235-2239
[3]   Raman dye-labeled nanoparticle probes for proteins [J].
Cao, YC ;
Jin, RC ;
Nam, JM ;
Thaxton, CS ;
Mirkin, CA .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (48) :14676-14677
[4]   Gold and silica-coated gold nanoparticles as thermographic labels for DNA detection [J].
Cerruti, Marta G. ;
Sauthier, Marc ;
Leonard, Donovan ;
Liu, Dage ;
Duscher, Gerard ;
Feldheim, Daniel L. ;
Franzen, Stefan .
ANALYTICAL CHEMISTRY, 2006, 78 (10) :3282-3288
[5]   Gold nanoparticles: Assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology [J].
Daniel, MC ;
Astruc, D .
CHEMICAL REVIEWS, 2004, 104 (01) :293-346
[6]   A fluorescence-based method for determining the surface coverage and hybridization efficiency of thiol-capped oligonucleotides bound to gold thin films and nanoparticles [J].
Demers, LM ;
Mirkin, CA ;
Mucic, RC ;
Reynolds, RA ;
Letsinger, RL ;
Elghanian, R ;
Viswanadham, G .
ANALYTICAL CHEMISTRY, 2000, 72 (22) :5535-5541
[7]   Temperature-programmed assembly of DNA:Au nanoparticle bioconjugates [J].
Dillenback, LM ;
Goodrich, GP ;
Keating, CD .
NANO LETTERS, 2006, 6 (01) :16-23
[8]   Role of cysteine and glutathione in HIV infection and other diseases associated with muscle wasting and immunological dysfunction [J].
Droge, W ;
Holm, E .
FASEB JOURNAL, 1997, 11 (13) :1077-1089
[9]   Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles [J].
Elghanian, R ;
Storhoff, JJ ;
Mucic, RC ;
Letsinger, RL ;
Mirkin, CA .
SCIENCE, 1997, 277 (5329) :1078-1081
[10]  
Goodman MT, 2000, CANCER-AM CANCER SOC, V89, P376, DOI 10.1002/1097-0142(20000715)89:2&lt