A DNA-gold nanoparticle-based colorimetric competition assay for the detection of cysteine
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作者:
Lee, Jae-Seung
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Northwestern Univ, Int Inst Nanotechnol, Dept Chem, Evanston, IL 60208 USANorthwestern Univ, Int Inst Nanotechnol, Dept Chem, Evanston, IL 60208 USA
Lee, Jae-Seung
[1
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Ulmann, Pirmin A.
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Northwestern Univ, Int Inst Nanotechnol, Dept Chem, Evanston, IL 60208 USANorthwestern Univ, Int Inst Nanotechnol, Dept Chem, Evanston, IL 60208 USA
Ulmann, Pirmin A.
[1
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Han, Min Su
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Northwestern Univ, Int Inst Nanotechnol, Dept Chem, Evanston, IL 60208 USANorthwestern Univ, Int Inst Nanotechnol, Dept Chem, Evanston, IL 60208 USA
Han, Min Su
[1
]
Mirkin, Chad A.
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Northwestern Univ, Int Inst Nanotechnol, Dept Chem, Evanston, IL 60208 USANorthwestern Univ, Int Inst Nanotechnol, Dept Chem, Evanston, IL 60208 USA
Mirkin, Chad A.
[1
]
机构:
[1] Northwestern Univ, Int Inst Nanotechnol, Dept Chem, Evanston, IL 60208 USA
We report the development of a highly sensitive and selective colorimetric detection method for cysteine based upon oligonucleotide-functionalized gold nanoparticle probes that contain strategically placed thymidine-thymidine (T-T) mismatches complexed with Hg2+. This assay relies upon the distance-dependent optical properties of gold nanoparticles, the sharp melting transition of oligonucleotide-linked nanoparticle aggregates, and the very selective coordination of Hg2+ with cysteine. The concentration of cysteine can be determined by monitoring with the naked eye or a UV-vis spectrometer the temperature at which the purple-to-red color change associated with aggregate dissociation takes place. This assay does not utilize organic cosolvents, enzymatic reactions, light-sensitive dye molecules, lengthy protocols, or sophisticated instrumentation thereby overcoming some of the limitations of more conventional methods.