A DNA-gold nanoparticle-based colorimetric competition assay for the detection of cysteine

被引：456

作者：

Lee, Jae-Seung ^{[1
]}

Ulmann, Pirmin A. ^{[1
]}

Han, Min Su ^{[1
]}

Mirkin, Chad A. ^{[1
]}

机构：

[1] Northwestern Univ, Int Inst Nanotechnol, Dept Chem, Evanston, IL 60208 USA

来源：

NANO LETTERS | 2008年 / 8卷 / 02期

关键词：

D O I：

10.1021/nl0727563

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report the development of a highly sensitive and selective colorimetric detection method for cysteine based upon oligonucleotide-functionalized gold nanoparticle probes that contain strategically placed thymidine-thymidine (T-T) mismatches complexed with Hg2+. This assay relies upon the distance-dependent optical properties of gold nanoparticles, the sharp melting transition of oligonucleotide-linked nanoparticle aggregates, and the very selective coordination of Hg2+ with cysteine. The concentration of cysteine can be determined by monitoring with the naked eye or a UV-vis spectrometer the temperature at which the purple-to-red color change associated with aggregate dissociation takes place. This assay does not utilize organic cosolvents, enzymatic reactions, light-sensitive dye molecules, lengthy protocols, or sophisticated instrumentation thereby overcoming some of the limitations of more conventional methods.

引用

页码：529 / 533

页数：5

共 67 条

[41] Theoretical studies of metal ion selectivity.: 1.: DFT calculations of interaction energies of amino acid side chains with selected transition metal ions (Co2+, Ni2+, Cu2+, Zn2+, Cd2+, and Hg2+) [J].

Rulísek, L ;

Havlas, Z .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (42) :10428-10439

[42] Visual detection of cysteine and homocysteine [J].

Rusin, O ;

St Luce, NN ;

Agbaria, RA ;

Escobedo, JO ;

Jiang, S ;

Warner, IM ;

Dawan, FB ;

Lian, K ;

Strongin, RM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (02) :438-439

[43] Effect of L-cysteine on some urinary risk factors in experimental hyperoxaluric rats [J].

Saravanan, N ;

Senthil, D ;

Varalakshmi, P .

BRITISH JOURNAL OF UROLOGY, 1996, 78 (01) :22-24

[44] Lead phthalocyanine as a selective carrier for preparation of a cysteine-selective electrode [J].

Shahrokhian, S .

ANALYTICAL CHEMISTRY, 2001, 73 (24) :5972-5978

[45] A spiropyran-based ensemble for visual recognition and quantification of cysteine and homocysteine at physiological levels [J].

Shao, Na ;

Jin, Jian Yu ;

Cheung, Sin Man ;

Yang, Rong Hua ;

Chan, Wing Hong ;

Mo, Tian .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (30) :4944-4948

[46] Multiplexed detection of protein cancer markers with biobarcoded nanoparticle probes [J].

Stoeva, Savka I. ;

Lee, Jae-Seung ;

Smith, Jennifer E. ;

Rosen, Steven T. ;

Mirkin, Chad A. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (26) :8378-8379

[47] Multiplexed DNA detection with biobarcoded nanoparticle probes [J].

Stoeva, Savka I. ;

Lee, Jae-Seung ;

Thaxton, C. Shad ;

Mirkin, Chad A. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (20) :3303-3306

[48] What controls the optical properties of DNA-linked gold nanoparticle assemblies? [J].

Storhoff, JJ ;

Lazarides, AA ;

Mucic, RC ;

Mirkin, CA ;

Letsinger, RL ;

Schatz, GC .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (19) :4640-4650

[49] One-pot colorimetric differentiation of polynucleotides with single base imperfections using gold nanoparticle probes [J].

Storhoff, JJ ;

Elghanian, R ;

Mucic, RC ;

Mirkin, CA ;

Letsinger, RL .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (09) :1959-1964

[50]

STRYER L, 1995, BIOCHEMISTRY-US, P21

← 1 2 3 4 5 6 7 →