Selected reaction monitoring applied to proteomics

被引：228

作者：

Gallien, Sebastien ^{[1
]}

Duriez, Elodie ^{[1
]}

Domon, Bruno ^{[1
]}

机构：

[1] Ctr Rech Publ Sante, Luxembourg Clin Prote Ctr, L-1445 Strassen, Luxembourg

来源：

JOURNAL OF MASS SPECTROMETRY | 2011年 / 46卷 / 03期

关键词：

selected reaction monitoring; proteomics; quantification; triple quadrupole mass spectrometer; multiplexing; TANDEM MASS-SPECTROMETRY; PEPTIDE IMMUNOAFFINITY ENRICHMENT; PROSTATE-SPECIFIC ANTIGEN; ABSOLUTE QUANTIFICATION; QUANTITATIVE-ANALYSIS; HUMAN PLASMA; PROTEOTYPIC PEPTIDES; MULTIPLEXED ASSAYS; ACCURATE MASS; HUMAN SERUM;

D O I：

10.1002/jms.1895

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Selected reaction monitoring (SRM) performed on triple quadrupole mass spectrometers has been the reference quantitative technique to analyze small molecules for several decades. It is now emerging in proteomics as the ideal tool to complement shotgun qualitative studies; targeted SRM quantitative analysis offers high selectivity, sensitivity and a wide dynamic range. However, SRM applied to proteomics presents singularities that distinguish it from small molecules analysis. This review is an overview of SRM technology and describes the specificities and the technical aspects of proteomics experiments. Ongoing developments aiming at increasing multiplexing capabilities of SRM are discussed; they dramatically improve its throughput and extend its field of application to directed or supervised discovery experiments. Copyright (C) 2011 John Wiley & Sons, Ltd.

引用

页码：298 / 312

页数：15

共 86 条

[51] A correlation algorithm for the automated quantitative analysis of shotgun proteomics data [J].

MacCoss, MJ ;

Wu, CC ;

Liu, HB ;

Sadygov, R ;

Yates, JR .

ANALYTICAL CHEMISTRY, 2003, 75 (24) :6912-6921

[52] Evaluation and optimization of ion-current ratio measurements by selected-ion-monitoring mass spectrometry [J].

MacCoss, MJ ;

Toth, MJ ;

Matthews, DE .

ANALYTICAL CHEMISTRY, 2001, 73 (13) :2976-2984

[53] eComputational prediction of proteotypic peptides for quantitative proteomics [J].

Mallick, Parag ;

Schirle, Markus ;

Chen, Sharon S. ;

Flory, Mark R. ;

Lee, Hookeun ;

Martin, Daniel ;

Raught, Brian ;

Schmitt, Robert ;

Werner, Thilo ;

Kuster, Bernhard ;

Aebersold, Ruedi .

NATURE BIOTECHNOLOGY, 2007, 25 (01) :125-131

[54] THE MECHANISMS OF COLLISIONAL ACTIVATION OF IONS IN MASS SPECTROMETRY [J].

Mayer, Paul M. ;

Poon, Clement .

MASS SPECTROMETRY REVIEWS, 2009, 28 (04) :608-639

[55]

McLafferty F.W., 1983, TANDEM MASS SPECTROM

[56]

Mirgorodskaya OA, 2000, RAPID COMMUN MASS SP, V14, P1226, DOI 10.1002/1097-0231(20000730)14:14<1226::AID-RCM14>3.3.CO

[57]

2-M

[58]

MURRAY KK, 2006, IUPAC RECOM IN PRESS

[59] Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics [J].

Ong, SE ;

Blagoev, B ;

Kratchmarova, I ;

Kristensen, DB ;

Steen, H ;

Pandey, A ;

Mann, M .

MOLECULAR & CELLULAR PROTEOMICS, 2002, 1 (05) :376-386

[60] A database of mass spectrometric assays for the yeast proteome [J].

Picotti, Paola ;

Lam, Henry ;

Campbell, David ;

Deutsch, Eric W. ;

Mirzaei, Hamid ;

Ranish, Jeff ;

Domon, Bruno ;

Aebersold, Ruedi .

NATURE METHODS, 2008, 5 (11) :913-914

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