The effect of local structural disruption on the yield of photochemical ligation between anthracene-oligonucleotide conjugates

被引：7

作者：

Arslan, Pelin ^{[1
]}

Ihara, Toshihiro ^{[1
,2
]}

Mukae, Motoko ^{[1
]}

Jyo, Akinori ^{[1
]}

机构：

[1] Kumamoto Univ, Grad Sch Sci & Technol, Dept Appl Chem & Biochem, Kumamoto 8608555, Japan

[2] Japan Sci & Technol Agcy, PRESTO, Kawaguchi, Saitama 3320012, Japan

来源：

ANALYTICAL SCIENCES | 2008年 / 24卷 / 01期

关键词：

D O I：

10.2116/analsci.24.173

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The techniques of chemical ligation have attracted great attention as an alternative to enzymatic joining of DNA ends. Here we introduce the photoligation of anthracene-modified ODN conjugates through anthracene cyclodimer formation. The effect of the positions and the kinds of single base mismatch on the template was evaluated using eight templates with one-base displacements. We found out that the yield of the ligation was affected by mispairing in a positiondependent manner. Such results would be attributed to the disruption of the local structure at the ligation site

引用

页码：173 / 176

页数：4

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