A third interferon-gamma-induced subunit exchange in the 20S proteasome

被引：140

作者：

Groettrup, M ^{[1
]}

Kraft, R ^{[1
]}

Kostka, S ^{[1
]}

Standera, S ^{[1
]}

Stohwasser, R ^{[1
]}

Kloetzel, PM ^{[1
]}

机构：

[1] MAX DELBRUCK CTR MOLEC MED,BERLIN,GERMANY

来源：

EUROPEAN JOURNAL OF IMMUNOLOGY | 1996年 / 26卷 / 04期

关键词：

proteasome; interferon-gamma; MECL-1; antigen presentation; major histocompatibility complex;

D O I：

10.1002/eji.1830260421

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The 20S proteasome is a protease complex of functional importance for antigen processing. Two of the 14 proteasome subunits, delta and MB1, can be replaced by the major histocompatibility complex (MHC)-encoded and interferon-gamma (IFN-gamma)-inducible subunits LMP2 and LMP7, respectively. LMP2 and LMP7 alter the cleavage site specificity of the 20S proteasome and are required for the efficient generation of T cell epitopes from a number of viral proteins and for optimal MHC class I cell surface expression. We compared the 20S proteasome subunit pattern from IFN-gamma-induced and non-induced mouse fibroblasts on two-dimensional gels and identified a third subunit exchange by microsequencing: the non-MHC-encoded subunit MECL-1 is induced by IFN-gamma and replaces a so-far barely characterized beta subunit designated 'MC14'. In analogy to LMP2 and LMP7, MECL-1 may be functional in MHC class I-restricted antigen presentation.

引用

页码：863 / 869

页数：7

共 56 条

[21] NUCLEOTIDE-SEQUENCE OF PUP1 ENCODING A PUTATIVE PROTEASOME SUBUNIT IN SACCHAROMYCES-CEREVISIAE [J].