Fluorescent microsphere technique to measure cerebral blood flow in the rat

被引：10

作者：

De Visscher, G.

Haseldonckx, M.

Flameng, W.

机构：

[1] Katholieke Univ Leuven, Lab Expt Cardiac Surg, B-3000 Louvain, Belgium

[2] Janssen Pharmaceut NV, Johnson & Johnson Pharmaceut Res & Dev, ADME Tox In vivo, B-2340 Beerse, Belgium

来源：

NATURE PROTOCOLS | 2006年 / 1卷 / 04期

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1038/nprot.2006.332

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The present protocol describes a method for parallel measurement of cerebral blood flow (CBF) using fluorescent microspheres and structural assessment of the same material. The method is based on the standard microsphere technique, embolizing capillaries proportional to the blood flow, but requires dissolution of the tissue to retrieve the microspheres. To link the blood flow to the tissue morphology we modified the technique to fluorescent microspheres, which are quantified in cryo- or vibratome sections, allowing structural analysis by, for example, immunohistochemistry or standard histology. The protocol takes 8 h 50 min, without pauses, to complete, but additional flow measurements or specific protocols can increase the time needed.

引用

页码：2162 / 2170

页数：9

共 16 条

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