Cross-reactive phage-displayed mimotopes lead to the discovery of mimicry between HSV-1 and a brain-specific protein

被引：15

作者：

Cortese, I

Capone, S

Luchetti, S

Cortese, R

Nicosia, A

机构：

[1] Ist Ric Biol Mol P Angeletti, I-00040 Pomezia, Italy

[2] Univ Roma Tor Vergata, Neurol Clin, Rome, Italy

[3] IRCSS, Clin S Lucia, I-00179 Rome, Italy

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2001年 / 113卷 / 01期

关键词：

multiple sclerosis; CSF; herpes simplex virus; autoantigen; phage library; mimotope;

D O I：

10.1016/S0165-5728(00)00398-2

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We previously reported the selection of several families of phage-displayed peptide mimics (mimotopes) recognized by oligoclonal immunoglobulins present in the CSF of multiple sclerosis (MS) patients. To search for the natural antigens recognized by these antibodies, anti-sera were raised against one of the mimotopes and used as a probe in ELISA. Western blotting and immunoprecipitation experiments. Anti-mimotope IgG were found to cross-react with an epitope shared by a brain-specific factor conserved from rodents to humans, and the surface glycoprotein gB of HSV-I. These findings support the hypothesis that common viral infections are the triggering agents of self-reactive CSF antibodies, whose role in MS still remains to be elucidated. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：119 / 128

页数：10

共 38 条

[1] Identification of peptides specific for cerebrospinal fluid antibodies in multiple sclerosis by using phage libraries [J].