Characterization of gene expression profiles of 3 different human oral squamous cell carcinoma cell lines with different invasion and metastatic capacities

被引:15
作者
Erdem, N. Fazil
Carlson, Eric R.
Gerard, David A.
机构
[1] Univ Tennessee, Dept Oral & Maxillofacial Surg, Grad Sch Med, Knoxville, TN USA
[2] Univ Tennessee, Inst Canc, Knoxville, TN USA
关键词
D O I
10.1016/j.joms.2007.12.036
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Purpose: The gene expression of 3 oral squamous cell carcinoma (OSCC) human cell lines, BHY, HN, and HSC-3, were studied based on their reported ability to invade adjacent bone or metastasize to cervical lymph nodes and/or distant organs. Materials and Methods: The characteristics of each cell line were confirmed on scid mice using micro-positron emission tomography (PET)/computerized tomography (CT) imaging techniques. Complimentary DNA (cDNA) microarray techniques were used to determine the gene expression profile differences between each of the three OSCC cell lines. Results: BHY, HN, and HSC-3 cell lines expressed 139, 214, and 128 up-regulated genes; and 117, 262, and 117 down-regulated genes, respectively. The clusterization of data showed that there are 13 genes that are up-regulated and 83 genes that are down-regulated in all 3 OSCC cell tines. Collection of genes organized by pathway may cause aggregate evaluation of anomalies. Thus the pathway analysis performed for each cell line based on cDNA microarray results showed BHY, HN, and HSC-3 cell lines to have 8, 10, and 3 up-regulated pathways and 3, 9, and 6 down-regulated pathways, respectively. Conclusions: This study showed that cDNA microarray analysis is an effective tool for mapping molecular signatures. With this technique it is possible to observe the entire genome of a malignant tumor so as to appreciate the simultaneous interactions among thousands of genes. (c) 2008 American Association of Oral and Maxillofacial Surgeons.
引用
收藏
页码:918 / 927
页数:10
相关论文
共 28 条
[21]   Genetic aberrations in oral or head and neck squamous cell carcinoma 2: chromosomal aberrations [J].
Scully, C ;
Field, JK ;
Tanzawa, H .
ORAL ONCOLOGY, 2000, 36 (04) :311-327
[22]   Rare amplicons implicate frequent deregulation of cell fate specification pathways in oral squamous cell carcinoma [J].
Snijders, AM ;
Schmidt, BL ;
Fridlyand, J ;
Dekker, N ;
Pinkel, D ;
Jordan, RCK ;
Albertson, DG .
ONCOGENE, 2005, 24 (26) :4232-4242
[23]   Indian hedgehog signaling regulates proliferation and differentiation of chondrocytes and is essential for bone formation [J].
St-Jacques, B ;
Hammerschmidt, M ;
McMahon, AP .
GENES & DEVELOPMENT, 1999, 13 (16) :2072-2086
[24]   Cell adhesion molecules and oral cancer [J].
Thomas, GJ ;
Speight, PM .
CRITICAL REVIEWS IN ORAL BIOLOGY & MEDICINE, 2001, 12 (06) :479-498
[25]   Regulation of matrix metalloproteinase expression in turner invasion [J].
Westermarck, J ;
Kähäri, VM .
FASEB JOURNAL, 1999, 13 (08) :781-792
[26]   Genomic alterations in oral squamous cell carcinoma cell lines detected by two-dimensional gel analysis [J].
Yamamoto, K ;
Konishi, N ;
Inui, T ;
Kitahori, Y ;
Hiasa, Y ;
Kirita, T ;
Sugimura, M .
ORAL ONCOLOGY, 1998, 34 (06) :508-512
[27]  
Yeh KT, 2003, INT J ONCOL, V23, P1001
[28]  
Zheng DQ, 1999, CANCER RES, V59, P1655