High throughput screening of enzyme activity with mass spectrometry imaging

被引：39

作者：

de Rond, Tristan ^{[1
]}

Danielewicz, Megan ^{[2
]}

Northen, Trent ^{[2
,3
]}

机构：

[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA

[3] Joint BioEnergy Inst, Emeryville, CA 94608 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2015年 / 31卷

基金：

美国国家科学基金会;

关键词：

TIME-OF-FLIGHT; COATED GLASS SLIDES; MALDI-TOF MS; POROUS SILICON; LASER-DESORPTION; HIGH-SENSITIVITY; PEPTIDE ARRAYS; ACTIVITY ASSAY; DESORPTION/IONIZATION; CHIP;

D O I：

10.1016/j.copbio.2014.07.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mass spectrometry imaging (MSI) has found a diversity of applications ranging from localizing metabolites and proteins in tissues to investigating microbial interactions, and as a result is perhaps the fastest growing subfield of mass spectrometry. Advances in surface mass spectrometry technologies are equally applicable to the analysis of arrayed samples. One promising field in which this capacity has been leveraged is the high-throughput analysis of enzyme activity, an important step in the development of a wide range of biotechnologies. This review article describes several emerging approaches that seek to improve the quality and scope of this application of MSI.

引用

页码：1 / 9

页数：9

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