High-throughput, nontargeted metabolite fingerprinting using nominal mass flow injection electrospray mass spectrometry

被引：100

作者：

Beckmann, Manfred ^{[1
]}

Parker, David ^{[1
]}

Enot, David P. ^{[1
]}

Duval, Emilie ^{[1
]}

Draper, John ^{[1
]}

机构：

[1] Univ Coll Wales Aberystwyth, Inst Biol Sci, Aberystwyth SY23 3DA, Ceredigion, Wales

来源：

NATURE PROTOCOLS | 2008年 / 3卷 / 03期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1038/nprot.2007.500

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Flow injection electrospray-mass spectrometry (FIE-MS) is finding utility as a first-pass metabolite fingerprinting tool in many research fields. We provide a protocol that has proved reliable in large-scale research projects involving diverse sample matrices originating from plants, microbes and mammalian biofluids. Using Brachypodium leaf material as an example matrix all steps are summarized from sample extraction to data quality assessment. Alternative procedures for dealing with other common matrices such as bloods and urine are included. With little sample pretreatment, no chromatography and instrument cycle times of <5 min it is feasible to analyze >1,000 samples per week. Analysis of a typical batch of 240 samples (including first-pass data analysis) can be accomplished within 48 h.

引用

页码：486 / 504

页数：19

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