Multispectral photoacoustic imaging of fluorochromes in small animals

被引：170

作者：

Razansky, Daniel ^{[1
]}

Vinegoni, Claudio ^{[1
]}

Ntziachristos, Vasilis ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Ctr Mol Imaging Res, Charlestown, MA 02129 USA

来源：

OPTICS LETTERS | 2007年 / 32卷 / 19期

关键词：

D O I：

10.1364/OL.32.002891

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Fluorochromes have become essential reporter molecules in biological research. We show that the depth-resolved distribution of fluorochromes in small animals can be imaged with 25 fmol sensitivity and 150 Am spatial resolution by means of multispectral photoacoustic imaging. The major advantage of the multispectral approach is the sensitive differentiation of chromophores and fluorochromes of interest based on self-reference measurements, as evidenced in this study by resolving a commonly used fluorochrome (Alexa Fluor 750) in mouse. The suggested method is well suited for enhancing visualization of functional and molecular information in vivo and longitudinally. (c) 2007 Optical Society of America.

引用

收藏

页码：2891 / 2893

页数：3

相关论文

共 11 条

[1] The frequency-dependent directivity of a planar Fabry-Perot polymer film ultrasound sensor [J].

Cox, Benjamin T. ;

Beard, Paul C. .

IEEE TRANSACTIONS ON ULTRASONICS FERROELECTRICS AND FREQUENCY CONTROL, 2007, 54 (02) :394-404

[2] Green fluorescent protein imaging of tumour growth, metastasis, and angiogenesis in mouse models [J].

Hoffman, RM .

LANCET ONCOLOGY, 2002, 3 (09) :546-556

[3] Thermoacoustic molecular imaging of small animals [J].

Kruger, Robert A. ;

Kiser, William L. ;

Reinecke, Daniel R. ;

Kruger, Gabe A. ;

Miller, Kathy D. .

Molecular Imaging, 2003, 2 (02) :113-123

[4] Quantitative spatially resolved measurement of tissue chromophore concentrations using photoacoustic spectroscopy: application to the measurement of blood oxygenation and haemoglobin concentration [J].

Laufer, Jan ;

Delpy, Dave ;

Elwell, Clare ;

Beard, Paul .

PHYSICS IN MEDICINE AND BIOLOGY, 2007, 52 (01) :141-168

[5] Photoacoustic imaging of lacZ gene expression in vivo [J].

Li, Li ;

Zemp, Roger J. ;

Lungu, Gina ;

Stoica, George ;

Wang, Lihong V. .

JOURNAL OF BIOMEDICAL OPTICS, 2007, 12 (02)

[6] Tomographic fluorescence mapping of tumor targets [J].

Montet, X ;

Ntziachristos, V ;

Grimm, J ;

Weissleder, R .

CANCER RESEARCH, 2005, 65 (14) :6330-6336

[7] Experimental three-dimensional fluorescence reconstruction of diffuse media by use of a normalized Born approximation [J].

Ntziachristos, V ;

Weissleder, R .

OPTICS LETTERS, 2001, 26 (12) :893-895

[8] Serial noninvasive photoacoustic imaging of neovascularization in tumor angiogenesis [J].

Siphanto, RI ;

Thumma, KK ;

Kolkman, RGM ;

van Leeuwen, TG ;

de Mul, FFM ;

van Neck, JW ;

van Adrichem, LNA ;

Steenbergen, W .

OPTICS EXPRESS, 2005, 13 (01) :89-95

[9] Noninvasive laser-induced photoacoustic tomography for structural and functional in vivo imaging of the brain [J].

Wang, XD ;

Pang, YJ ;

Ku, G ;

Xie, XY ;

Stoica, G ;

Wang, LHV .

NATURE BIOTECHNOLOGY, 2003, 21 (07) :803-806

[10] Photoacoustic tomography of a nanoshell contrast agent in the in vivo rat brain [J].

Wang, YW ;

Xie, XY ;

Wang, XD ;

Ku, G ;

Gill, KL ;

O'Neal, DP ;

Stoica, G ;

Wang, LV .

NANO LETTERS, 2004, 4 (09) :1689-1692