The prospects for designer single-stranded RNA-binding proteins

被引：50

作者：

Mackay, Joel P. ^{[1
]}

Font, Josep ^{[1
]}

Segal, David J. ^{[2
,3
]}

机构：

[1] Univ Sydney, Sch Mol Biosci, Sydney, NSW 2006, Australia

[2] Univ Calif Davis, Dept Pharmacol, Davis, CA 95616 USA

[3] Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2011年 / 18卷 / 03期

基金：

英国医学研究理事会;

关键词：

ZINC-FINGER NUCLEASES; STRUCTURAL BASIS; KH DOMAINS; NUCLEOCAPSID PROTEIN; MOLECULAR-BASIS; TRACT RECOGNITION; CRYSTAL-STRUCTURE; SPECIFICITY; REVEALS; DNA;

D O I：

10.1038/nsmb.2005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Spectacular progress has been made in the design of proteins that recognize double-stranded DNA with a chosen specificity, to the point that designer DNA-binding proteins can be ordered commercially. This success raises the question of whether it will be possible to engineer libraries of proteins that can recognize RNA with tailored specificity. Given the recent explosion in the number and diversity of RNA species demonstrated to play roles in biology, designer RNA-binding proteins are set to become valuable tools, both in the research laboratory and potentially in the clinic. Here we discuss the prospects for the realization of this idea.

引用

页码：256 / 261

页数：6

共 58 条

[11] Zinc-finger nucleases: The next generation emerges [J].

Cathomen, Toni ;

Joung, J. Keith .

MOLECULAR THERAPY, 2008, 16 (07) :1200-1207

[12] Protein families and RNA recognition [J].

Chen, Y ;

Varani, G .

FEBS JOURNAL, 2005, 272 (09) :2088-2097

[13] Cell-free selection of RNA-binding proteins using in vitro compartmentalization [J].

Chen, Yu ;

Mandic, Jana ;

Varani, Gabriele .

NUCLEIC ACIDS RESEARCH, 2008, 36 (19)

[14] Engineering RNA sequence specificity of Pumilio repeats [J].

Cheong, Cheorn-Gil ;

Hall, Traci M. Tanaka .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (37) :13635-13639

[15] Distinct contributions of KH domains to substrate binding affinity of Drosophila P-element somatic inhibitor protein [J].

Chmiel, NH ;

Rio, DC ;

Doudna, JA .

RNA, 2006, 12 (02) :283-291

[16] RNA recognition motifs:: boring?: Not quite [J].

Clery, Antoine ;

Blatter, Markus ;

Allain, Frederic H-T .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2008, 18 (03) :290-298

[17] mRNA stabilization by poly(A) binding protein is independent of poly(A) and requires translation [J].

Coller, JM ;

Gray, NK ;

Wickens, MP .

GENES & DEVELOPMENT, 1998, 12 (20) :3226-3235

[18] Translation driven by an eIF4G core domain in vivo [J].

De Gregorio, E ;

Preiss, T ;

Hentze, MW .

EMBO JOURNAL, 1999, 18 (17) :4865-4874

[19] Structure of the HIV-1 nucleocapsid protein bound to the SL3 Ψ-RNA recognition element [J].

De Guzman, RN ;

Wu, ZR ;

Stalling, CC ;

Pappalardo, L ;

Borer, PN ;

Summers, MF .

SCIENCE, 1998, 279 (5349) :384-388

[20] Composition and sequence-dependent binding of RNA to the nucleocapsid protein of Moloney murine leukemia virus [J].

Dey, A ;

York, D ;

Smalls-Mantey, A ;

Summers, MF .

BIOCHEMISTRY, 2005, 44 (10) :3735-3744

← 1 2 3 4 5 6 →