High-throughput sequencing reveals the diversity of TCR β chain CDR3 repertoire in patients with severe acne

被引：6

作者：

Shao, Lei ^{[1
,2
]}

Liu, Yumei ^{[1
,2
]}

Mei, Junpu ^{[3
]}

Li, Dongmei ^{[4
]}

Chen, Lijie ^{[1
,2
]}

Pan, Qingli ^{[1
,2
]}

Zhang, Shujuan ^{[1
,2
]}

Dai, Xiangnong ^{[1
,2
]}

Liang, Jingyao ^{[1
,2
]}

Sun, Silong ^{[3
]}

Wang, Jianqin ^{[1
,2
]}

机构：

[1] Guangzhou Med Univ, Inst Dermatol, Guangzhou 510095, Peoples R China

[2] Guangzhou Inst Dermatol, Dept Dermatol, 56 Hengfu Rd, Guangzhou 510095, Peoples R China

[3] BGI Shenzhen, BGI Genom, Shenzhen 518083, Peoples R China

[4] Georgetown Univ, Med Ctr, Dept Microbiol & Immunol, Washington, DC 20007 USA

来源：

MOLECULAR IMMUNOLOGY | 2020年 / 120卷

关键词：

Acne vulgaris; Repertoire; TCR; CDR3; High-throughput sequencing; PROPIONIBACTERIUM-ACNES; INNATE IMMUNITY; EPIDEMIOLOGY; INFLAMMATION; INDIVIDUALS; CELLS; INDEX;

D O I：

10.1016/j.molimm.2020.01.024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Acne is a common chronic inflammatory skin disease, and the inflammation immune response runs through all stages of acne lesions. In this study, we use a combination of multiplex-PCR and high-throughput sequencing technologies to analyze T cell receptor beta chain CDR3 (complementarity-determining region 3) in peripheral blood isolated from severe acne patients. Once compared with healthy controls, we propose to identify acne-relevant CDR3 peptides. Our results reveal that the diversity of T cell receptor beta chain (TRB) CDR3 sequences in the peripheral blood of the severe acne vulgaris (SA) group differed from that of the control group. In addition, we find 10 TRB CDR3 sequences, amino acid sequences and V-J combinations with significantly different expressions between the SA group and the non-acne (NA) group (P< 0.0001). These findings may contribute to a better understanding of the role of immunity in the pathogenesis of acne and may serve as biomarkers for evaluating risk or prognosis of severe acne disease in future.

引用

页码：23 / 31

页数：9

共 35 条

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