Short-wavelength two-photon excitation fluorescence microscopy of tryptophan with a photonic crystal fiber based light source

被引：43

作者：

Palero, JA ^{[1
]}

Boer, VO

Vijverberg, JC

Gerritsen, HC

Sterenborg, HJCM

机构：

[1] Univ Utrecht, NL-3584 CC Utrecht, Netherlands

[2] Erasmus Univ, Med Ctr, Ctr Opt Diagnost & Therapy, NL-3000 CA Rotterdam, Netherlands

来源：

OPTICS EXPRESS | 2005年 / 13卷 / 14期

关键词：

D O I：

10.1364/OPEX.13.005363

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We report on a novel and simple light source for short-wavelength two-photon excitation fluorescence microscopy based on the visible nonsolitonic radiation from a photonic crystal fiber. We demonstrate tunability of the light source by varying the wavelength and intensity of the Ti:Sapphire excitation light source. The visible nonsolitonic radiation is used as an excitation light source for two-photon fluorescence microscopy of tryptophan powder. (C) 2005 Optical Society of America.

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收藏

页码：5363 / 5368

页数：6

相关论文

共 23 条

[11] Experimental evidence for supercontinuum generation by fission of higher-order solitons in photonic fibers [J].

Herrmann, J ;

Griebner, U ;

Zhavoronkov, N ;

Husakou, A ;

Nickel, D ;

Knight, JC ;

Wadsworth, WJ ;

Russell, PSJ ;

Korn, G .

PHYSICAL REVIEW LETTERS, 2002, 88 (17) :1739011-1739014

[12] Initial steps of supercontinuum generation in photonic crystal fibers [J].

Hilligsoe, KM ;

Paulsen, HN ;

Thogersen, J ;

Keiding, SR ;

Larsen, JJ .

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS, 2003, 20 (09) :1887-1893

[13] Supercontinuum generation, four-wave mixing, and fission of higher-order solitons in photonic-crystal fibers [J].

Husakou, AV ;

Herrmann, J .

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS, 2002, 19 (09) :2171-2182

[14] Multi-spectral two-photon excited fluorescence microscopy using supercontinuum light source [J].

Isobe, K ;

Watanabe, W ;

Matsunaga, S ;

Higashi, T ;

Fukui, K ;

Itoh, K .

JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS & EXPRESS LETTERS, 2005, 44 (1-7) :L167-L169

[15] Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with fura-2 [J].

McConnell, G ;

Riis, E .

PHYSICS IN MEDICINE AND BIOLOGY, 2004, 49 (20) :4757-4763

[16] Confocal laser scanning fluorescence microscopy with a visible continuum source [J].

McConnell, G .

OPTICS EXPRESS, 2004, 12 (13) :2844-2850

[17] Pulse breaking and supercontinuum generation with 200-fs pump pulses in photonic crystal fibers [J].

Ortigosa-Blanch, A ;

Knight, JC ;

Russell, PSJ .

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS, 2002, 19 (11) :2567-2572

[18] Coherent anti-Stokes Raman scattering microscopy with a photonic crystal fiber based light source [J].

Paulsen, HN ;

Hilligsoe, KM ;

Thogersen, J ;

Keiding, SR ;

Larsen, JJ .

OPTICS LETTERS, 2003, 28 (13) :1123-1125

[19] 3-DIMENSIONALLY RESOLVED NAD(P)H CELLULAR METABOLIC REDOX IMAGING OF THE IN-SITU CORNEA WITH 2-PHOTON EXCITATION LASER-SCANNING MICROSCOPY [J].

PISTON, DW ;

MASTERS, BR ;

WEBB, WW .

JOURNAL OF MICROSCOPY, 1995, 178 :20-27

[20] Optical properties of high-delta air-silica microstructure optical fibers [J].

Ranka, JK ;

Windeler, RS ;

Stentz, AJ .

OPTICS LETTERS, 2000, 25 (11) :796-798