Immunoprecipitation of native chromatin: NChIP

被引：142

作者：

O'Neill, LP ^{[1
]}

Turner, BM ^{[1
]}

机构：

[1] Univ Birmingham, Sch Med, Dept Anat, Chromatin & Gene Express Grp, Birmingham B15 2TT, W Midlands, England

来源：

METHODS | 2003年 / 31卷 / 01期

关键词：

chromatin; nucleosome; immunoprecipitation; modified histones; anti-histone antibodies; DNA slot blotting;

D O I：

10.1016/S1046-2023(03)00090-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Chromatin immunoprecipitation (ChIP) is widely used in many fields to analyze the distribution of specific proteins, or their modified isoforms, across defined DNA domains. ChIP procedures fall into two main categories, namely, those that use native chromatin prepared by nuclease digestion (designated NChIP), and those that use chromatin in which DNA and proteins are crosslinked, either chemically or with UV light (designated XChIP). Each procedure has its own advantages and drawbacks. Here, we outline the methods currently in use in our laboratory to isolate and immunoprecipitate native chromatin from cultured cells, and to isolate and analyze immunoprecipitated protein and DNA. (C) 2003 Elsevier Science (USA). All rights reserved.

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页码：76 / 82

页数：7

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