Biosensors in chemical separations

被引：31

作者：

Fishman, HA ^{[1
]}

Greenwald, DR ^{[1
]}

Zare, RN ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

来源：

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE | 1998年 / 27卷

关键词：

capillary electrophoresis; chromatography; molecular recognition; drug-discovery; patch clamp;

D O I：

10.1146/annurev.biophys.27.1.165

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Identification of biomolecules in complex biological mixtures represents a major challenge in biomedical, environmental, and chemical research today. Chemical separations with traditional detection schemes such as absorption, fluorescence, refractive index, conductivity, and electrochemistry have been the standards for definitive identifications of many compounds. In many instances, however, the complexity of the biomixture exceeds the resolution capability of chemical separations. Biosensors based on molecular recognition can dramatically improve the selectivity of and provide biologically relevant information about the components. This review describes how coupling chemical separations with online biosensors solves challenging problems in sample analysis by identifying components that would not normally be detectable by either technique alone. This review also presents examples and principles of combining chemical separations with biosensor detection that uses living systems, whole cells, membrane receptors, enzymes, and immunosensors.

引用

页码：165 / 198

页数：34

共 119 条

[71] DETERMINATION OF ORGANIC-ACIDS, AMINO-ACIDS AND SACCHARIDES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND A POSTCOLUMN ENZYME REACTOR WITH AMPEROMETRIC DETECTION [J].