Expression of a CIC chloride channel in Caenorhabditis elegans gamma-aminobutyric acid-ergic neurons

被引：10

作者：

Bianchi, L

Miller, DM

George, AL

机构：

[1] Vanderbilt Univ, Sch Med, Dept Med, Div Med Genet, Nashville, TN 37232 USA

[2] Vanderbilt Univ, Sch Med, Dept Pharmacol, Nashville, TN 37212 USA

[3] Vanderbilt Univ, Sch Med, Dept Cell Biol, Nashville, TN 37212 USA

来源：

NEUROSCIENCE LETTERS | 2001年 / 299卷 / 03期

关键词：

CIC channels; Caenorhabditis elegans; gamma-aminobutyric acid-ergic neurons; green fluorescent protein fusions;

D O I：

10.1016/S0304-3940(01)01525-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

In this study, we report the complete cDNA sequence, genomic organization and expression pattern of the Caenorhabditis elegans CIC chloride channel gene, clh-6. Two different types of reporter gene fusions suggest that clh-6 expression is restricted to two gamma-aminobutyric acid-ergic RME neurons. These results are in striking contrast with the wide tissue distribution of messenger RNA for the related mammalian isoforms, CIC-6 a nd CIC-7. The restricted expression pattern of clh-6 provides a unique opportunity to study the biological function of a neuronal CIC chloride channel. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

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收藏

页码：177 / 180

页数：4

相关论文

共 20 条

[1] Anion channels in higher plants: functional characterization, molecular structure and physiological role [J].

Barbier-Brygoo, H ;

Vinauger, M ;

Colcombet, J ;

Ephritikhine, G ;

Frachisse, JM ;

Maurel, C .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2000, 1465 (1-2) :199-218

[2] Neurobiology of the Caenorhabditis elegans genome [J].

Bargmann, CI .

SCIENCE, 1998, 282 (5396) :2028-2033

[3] Nematode spliced leaders - Ubiquity, evolution and utility [J].

Blaxter, M ;

Liu, L .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1996, 26 (10) :1025-1033

[4] ClC-6 and ClC-7 are two novel broadly expressed members of the CLC chloride channel family [J].

Brandt, S ;

Jentsch, TJ .

FEBS LETTERS, 1995, 377 (01) :15-20

[5] Evidence for the intracellular location of chloride channel (CIC)-type proteins:: co-localization of CIC-6a and CIC-6c with the sarco/endoplasmic-reticulum Ca2+ pump SERCA2b [J].

Buyse, G ;

Trouet, D ;

Voets, T ;

Missiaen, L ;

Droogmans, G ;

Nilius, B ;

Eggermont, J .

BIOCHEMICAL JOURNAL, 1998, 330 :1015-1021

[6] Genome sequence of the nematode C-elegans:: A platform for investigating biology [J].

不详 .

SCIENCE, 1998, 282 (5396) :2012-2018

[7] The yeast CLC chloride channel functions in cation homeostasis [J].

Gaxiola, RA ;

Yuan, DS ;

Klausner, RD ;

Fink, GR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (07) :4046-4050

[8] Chloride channels and endocytosis: ClC-5 makes a dent [J].

George, AL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (14) :7843-7845

[9] A VOLTAGE-GATED CHLORIDE CHANNEL IN THE YEAST SACCHAROMYCES-CEREVISIAE [J].

HUANG, ME ;

CHUAT, JC ;

GALIBERT, F .

JOURNAL OF MOLECULAR BIOLOGY, 1994, 242 (04) :595-598

[10] Complete genomic structure of the CLCN6 and CLCN7 putative chloride channel genes [J].

Kornak, U ;

Bösl, MR ;

Kubisch, C .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1999, 1447 (01) :100-106