Recent advances in immobilization methods of antibodies on solid supports

被引：211

作者：

Jung, Yongwon ^{[1
]}

Jeong, Jin Young ^{[1
,2
]}

Chung, Bong Hyun ^{[1
,2
]}

机构：

[1] Korea Res Inst Biosci & Biotechnol, BioNanotechnol Res Ctr, Taejon 305600, South Korea

[2] Korea Univ Sci & Technol, Sch Engn, Taejon 305333, South Korea

来源：

ANALYST | 2008年 / 133卷 / 06期

关键词：

D O I：

10.1039/b800014j

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Antibody immobilization on a solid support is an essential process for the development of most immune-based assay systems. The choice of the immobilization method greatly affects antibody-antigen interactions on the assay surface. For the past several years, numerous strategies have been reported to control antibody immobilization, mainly by directing the orientation, stability, and density of bound antibodies on different assay platforms. Here we discuss recent developments in antibody immobilization methods with a particular focus on the strengths and limitations of reported approaches, and thereby provide a useful guideline for the selection of suitable antibody coupling procedures.

引用

页码：697 / 701

页数：5

共 36 条

[1] Profiling of differential protein expression in angiogenin-induced HUVECs using antibody-arrayed ProteoChip [J].

Ahn, EH ;

Kang, DK ;

Chang, SI ;

Kang, CS ;

Han, MH ;

Kang, IC .

PROTEOMICS, 2006, 6 (04) :1104-1109

[2] DNA-encoded antibody libraries: A unified platform for multiplexed cell sorting and detection of genes and proteins [J].

Bailey, Ryan C. ;

Kwong, Gabriel A. ;

Radu, Caius G. ;

Witte, Owen N. ;

Heath, James R. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (07) :1959-1967

[3] Protein-sensing assay formats and devices [J].

Bilitewski, Ursula .

ANALYTICA CHIMICA ACTA, 2006, 568 (1-2) :232-247

[4] DNA-directed protein immobilization for simultaneous detection of multiple analytes by surface plasmon resonance biosensor [J].

Boozer, C ;

Ladd, J ;

Chen, SF ;

Jiang, ST .

ANALYTICAL CHEMISTRY, 2006, 78 (05) :1515-1519

[5] DNA directed protein immobilization on mixed ssDNA/oligo(ethylene glycol) self-assembled monolayers for sensitive biosensors [J].

Boozer, C ;

Ladd, J ;

Chen, SF ;

Yu, Q ;

Homola, J ;

Jiang, SY .

ANALYTICAL CHEMISTRY, 2004, 76 (23) :6967-6972

[6]

Butler J. E., 1993, Molecular Immunology, V30, P1165, DOI 10.1016/0161-5890(93)90135-X

[7] Investigation of spacer length effect on immobilized Escherichia coli pili-antibody molecular recognition by AFM [J].

Cao, Ting ;

Wang, Anfeng ;

Liang, Xuemei ;

Tang, Haiying ;

Auner, Gregory W. ;

Salley, Steven O. ;

Ng, K. Y. Simon .

BIOTECHNOLOGY AND BIOENGINEERING, 2007, 98 (06) :1109-1122

[8] Fabrication and characterization of 3D hydrogel microarrays to measure antigenicity and antibody functionality for biosensor applications [J].

Charles, PT ;

Goldman, ER ;

Rangasammy, JG ;

Schauer, CL ;

Chen, MS ;

Taitt, CR .

BIOSENSORS & BIOELECTRONICS, 2004, 20 (04) :753-764

[9] Site-directed biotinylation of antibodies for controlled immobilization of solid surfaces [J].

Cho, Il-Hoon ;

Paek, Eui-Hwan ;

Lee, Haiwort ;

Kang, Ji Yoon ;

Kim, Tae Song ;

Paek, Se-Hwan .

ANALYTICAL BIOCHEMISTRY, 2007, 365 (01) :14-23

[10] Comparison of antibody functionality using different immobilization methods [J].

Danczyk, R ;

Krieder, B ;

North, A ;

Webster, T ;

HogenEsch, H ;

Rundell, A .

BIOTECHNOLOGY AND BIOENGINEERING, 2003, 84 (02) :215-223

← 1 2 3 4 →