Thermoresponsive giant biohybrid amphiphiles

被引：57

作者：

Lavigueur, Christine ^{[2
]}

Garcia, Jordi Gonzalez ^{[2
]}

Hendriks, Linda ^{[2
]}

Hoogenboom, Richard ^{[2
]}

Cornelissen, Jeroen J. L. M. ^{[1
]}

Nolte, Roeland J. M. ^{[2
]}

机构：

[1] Univ Twente, Lab Biomol Nanotechnol, MESA Inst Nanotechnol, NL-7500 AE Enschede, Netherlands

[2] Radboud Univ Nijmegen, Inst Mol & Mat, Dept Organ Chem, NL-6500 GL Nijmegen, Netherlands

来源：

POLYMER CHEMISTRY | 2011年 / 2卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

TRANSFER RADICAL POLYMERIZATION; COFACTOR RECONSTITUTION; CYCLOADDITION REACTION; AGGREGATION BEHAVIOR; RAFT POLYMERIZATION; BLOCK-COPOLYMERS; CLICK CHEMISTRY; SMART POLYMERS; PROTEIN; MICROGELS;

D O I：

10.1039/c0py00229a

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

A series of random copolymers of various lengths was prepared by atom transfer radical polymerisation (ATRP) using two acrylate monomers with short pendant ethylene glycol side chains (ethylene glycol methyl ether acrylate, EGMEA, and methoxy ethoxy ethyl acrylate, MEEA). The end group was converted to an azide to enable bioconjugation through copper-catalysed azide-alkyne cycloaddition (CuAAC). All polymers were found to be thermoresponsive, with a cloud point between 25 and 35 degrees C depending on their molecular weight. They were conjugated to enhanced green fluorescent protein (EGFP) functionalised with a single alkyne moiety, as seen by fast performance liquid chromatography (FPLC) and gel electrophoresis (SDS-PAGE). The resulting biohybrid amphiphiles were thermoresponsive. Dynamic light scattering (DLS) and transmission electron microscopy (TEM) were used to study their self-assembly at elevated temperatures, and they were found to form spherical structures with a diameter of approximately 60 nm upon slow heating.

引用

页码：333 / 340

页数：8

共 52 条

[1] Libraries of Methacrylic Acid and Oligo(ethylene glycol) Methacrylate Copolymers with LCST Behavior [J].

Becer, C. Remzi ;

Hahn, Sabine ;

Fijten, Martin W. M. ;

Thijs, Hanneke M. L. ;

Hoogenboom, Richard ;

Schubert, Ulrich S. .

JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, 2008, 46 (21) :7138-7147

[2] Self-Assembled Block Copolymer Aggregates: From Micelles to Vesicles and their Biological Applications [J].

Blanazs, Adam ;

Armes, Steven P. ;

Ryan, Anthony J. .

MACROMOLECULAR RAPID COMMUNICATIONS, 2009, 30 (4-5) :267-277

[3] Aggregation Behavior of giant amphiphiles prepared by cofactor reconstitution [J].

Boerakker, Mark J. ;

Botterhuis, Nicole E. ;

Bomans, Paul H. H. ;

Frederik, Peter M. ;

Meijer, Emmo M. ;

Nolte, Roeland J. M. ;

Sommerdijk, Nico A. J. M. .

CHEMISTRY-A EUROPEAN JOURNAL, 2006, 12 (23) :6071-6080

[4]

Boerakker MJ, 2002, ANGEW CHEM INT EDIT, V41, P4239, DOI 10.1002/1521-3773(20021115)41:22<4239::AID-ANIE4239>3.0.CO

[5]

2-E

[6] Self-assembly of polypeptide-based block copolymer amphiphiles [J].

Carlsen, Autumn ;

Lecommandoux, Sebastein .

CURRENT OPINION IN COLLOID & INTERFACE SCIENCE, 2009, 14 (05) :329-339

[7] Polytriazoles as copper(I)-stabilizing ligands in catalysis [J].

Chan, TR ;

Hilgraf, R ;

Sharpless, KB ;

Fokin, VV .

ORGANIC LETTERS, 2004, 6 (17) :2853-2855

[8] Polystyrene-b-poly(acrylic acid) vesicle size control using solution properties and hydrophilic block length [J].

Choucair, A ;

Lavigueur, C ;

Eisenberg, A .

LANGMUIR, 2004, 20 (10) :3894-3900

[9] Functional polymers by atom transfer radical polymerization [J].

Coessens, V ;

Pintauer, T ;

Matyjaszewski, K .

PROGRESS IN POLYMER SCIENCE, 2001, 26 (03) :337-377

[10] Temperature-regulated activity of responsive polymer-protein conjugates prepared by grafting-from via RAFT polymerization [J].

De, Priyadarsi ;

Li, Ming ;

Gondi, Sudershan R. ;

Sumerlin, Brent S. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (34) :11288-+

← 1 2 3 4 5 6 →