A new approach to manipulate the fate of single neural stem cells in tissue

被引：25

作者：

Taverna, Elena ^{[1
]}

Haffner, Christiane ^{[1
]}

Pepperkok, Rainer ^{[2
]}

Huttner, Wieland B. ^{[1
]}

机构：

[1] Max Planck Inst Mol Cell Biol & Genet, Dresden, Germany

[2] European Mol Biol Lab, Cell Biol & Biophys Unit, Heidelberg, Germany

来源：

NATURE NEUROSCIENCE | 2012年 / 15卷 / 02期

基金：

欧洲研究理事会;

关键词：

RADIAL GLIA; IN-VIVO; NEUROEPITHELIAL CELLS; DEVELOPING NEOCORTEX; BASAL PROGENITORS; CEREBRAL-CORTEX; SELF-RENEWAL; NEURONS; NEUROGENESIS; EXPANSION;

D O I：

10.1038/nn.3008

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A challenge in the field of neural stem cell biology is the mechanistic dissection of single stem cell behavior in tissue. Although such behavior can be tracked by sophisticated imaging techniques, current methods of genetic manipulation do not allow researchers to change the level of a defined gene product on a truly acute time scale and are limited to very few genes at a time. To overcome these limitations, we established microinjection of neuroepithelial/radial glial cells (apical progenitors) in organotypic slice culture of embryonic mouse brain. Microinjected apical progenitors showed cell cycle parameters that were indistinguishable to apical progenitors in utero, underwent self-renewing divisions and generated neurons. Microinjection of single genes, recombinant proteins or complex mixtures of RNA was found to elicit acute and defined changes in apical progenitor behavior and progeny fate. Thus, apical progenitor microinjection provides a new approach to acutely manipulating single neural stem and progenitor cells in tissue.

引用

页码：329 / 337

页数：9

共 44 条

[1] Neurons derive from the more apical daughter in asymmetric divisions in the zebrafish neural tube [J].

Alexandre, Paula ;

Reugels, Alexander M. ;

Barker, David ;

Blanc, Eric ;

Clarke, Jonathan D. W. .

NATURE NEUROSCIENCE, 2010, 13 (06) :673-U38

[2]

Alfei L, 1999, EUR J HISTOCHEM, V43, P29

[3] Neural stem and progenitor cells shorten S-phase on commitment to neuron production [J].

Arai, Yoko ;

Pulvers, Jeremy N. ;

Haffner, Christiane ;

Schilling, Britta ;

Nuesslein, Ina ;

Calegari, Federico ;

Huttner, Wieland B. .

NATURE COMMUNICATIONS, 2011, 2

[4] In Vivo Fate Mapping and Expression Analysis Reveals Molecular Hallmarks of Prospectively Isolated Adult Neural Stem Cells [J].

Beckervordersandforth, Ruth ;

Tripathi, Pratibha ;

Ninkovic, Jovica ;

Bayam, Efil ;

Lepier, Alexandra ;

Stempfhuber, Barbara ;

Kirchhoff, Frank ;

Hirrlinger, Johannes ;

Haslinger, Anja ;

Lie, D. Chichung ;

Beckers, Johannes ;

Yoder, Bradley ;

Irmler, Martin ;

Goetz, Magdalena .

CELL STEM CELL, 2010, 7 (06) :744-758

[5] The Rho-GTPase cdc42 regulates neural progenitor fate at the apical surface [J].

Cappello, Silvia ;

Attardo, Alessio ;

Wu, Xunwei ;

Iwasato, Takuji ;

Itohara, Shigeyoshi ;

Wilsch-Braeuninger, Michaela ;

Eilken, Hanna M. ;

Rieger, Michael A. ;

Schroeder, Timm T. ;

Huttner, Wieland B. ;

Brakebusch, Cord ;

Goetz, Magdalena .

NATURE NEUROSCIENCE, 2006, 9 (09) :1099-1107

[6] Niche-independent symmetrical self-renewal of a mammalian tissue stem cell [J].

Conti, L ;

Pollard, SM ;

Gorba, T ;

Reitano, E ;

Toselli, M ;

Biella, G ;

Sun, YR ;

Sanzone, S ;

Ying, QL ;

Cattaneo, E ;

Smith, A .

PLOS BIOLOGY, 2005, 3 (09) :1594-1606

[7] Pax6, Tbr2, and Tbr1 are expressed sequentially by radial glia, intermediate progenitor cells, and postmitotic neurons in developing neocortex [J].

Englund, C ;

Fink, A ;

Lau, C ;

Pham, D ;

Daza, RAM ;

Bulfone, A ;

Kowalczyk, T ;

Hevner, RF .

JOURNAL OF NEUROSCIENCE, 2005, 25 (01) :247-251

[8] Insulinoma-Associated 1 Has a Panneurogenic Role and Promotes the Generation and Expansion of Basal Progenitors in the Developing Mouse Neocortex [J].

Farkas, Lilla M. ;

Haffner, Christiane ;

Giger, Thomas ;

Khaitovich, Philipp ;

Nowick, Katja ;

Birchmeier, Carmen ;

Paeaebo, Svante ;

Huttner, Wieland B. .

NEURON, 2008, 60 (01) :40-55

[9] The cell biology of neural stem and progenitor cells and its significance for their proliferation versus differentiation during mammalian brain development [J].

Farkas, Lilla M. ;

Huttner, Wieland B. .

CURRENT OPINION IN CELL BIOLOGY, 2008, 20 (06) :707-715

[10] Cortical progenitor expansion, self-renewal and neurogenesis a polarized perspective [J].

Fietz, Simone A. ;

Huttner, Wieland B. .

CURRENT OPINION IN NEUROBIOLOGY, 2011, 21 (01) :23-35

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