Synthesis of a monocharged peptide nucleic acid (PNA) analog and its recognition as substrate by DNA polymerases

被引：6

作者：

Lutz, MJ

Will, DW

Breipohl, G

Benner, SA

Uhlmann, E

机构：

[1] Hoechst Marion Roussel Deutschland GMBH, Chem Res, D-65926 Frankfurt, Germany

[2] Swiss Fed Inst Technol, Dept Chem, CH-8092 Zurich, Switzerland

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 1999年 / 18卷 / 03期

关键词：

D O I：

10.1080/15257779908043084

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The preparation of a novel phosphoramidite monomer based on thyminyl acetic acid coup led to the secondary nitrogen of 2-(2-amino-ethylamino)ethanol is described. This monomer can be used to attach a deoxynucleotide to the carboxy terminus of a PNA oligomer by solid-phase synthesis. The resulting PNA primer is recognized as a substrate by various DNA polymerases.

引用

页码：393 / 401

页数：9

共 14 条

[11]

UHLMANN E, 1996, ANGEW CHEM, V108, P2793

[12] Optimization of the binding properties of PNA-(5′)-DNA chimerae [J].