Global effects of the small RNA biogenesis machinery on the Arabidopsis thaliana transcriptome

被引：56

作者：

Laubinger, Sascha ^{[1
,2
]}

Zeller, Georg ^{[1
,3
]}

Henz, Stefan R. ^{[1
]}

Buechel, Sabine ^{[1
,2
]}

Sachsenberg, Timo ^{[1
]}

Wang, Jia-Wei ^{[1
]}

Raetsch, Gunnar ^{[3
]}

Weigel, Detlef ^{[1
]}

机构：

[1] Max Planck Inst Dev Biol, Dept Mol Biol, D-72076 Tubingen, Germany

[2] Univ Tubingen, Ctr Plant Mol Biol ZMBP, D-72076 Tubingen, Germany

[3] Max Planck Gesell, Friedrich Miescher Inst, D-72076 Tubingen, Germany

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2010年 / 107卷 / 41期

关键词：

dicer-like; DNA methylation; miRNA; transposon; DICER-LIKE PROTEINS; STRESS-REGULATED MICRORNAS; TRANS-ACTING SIRNAS; DNA METHYLATION; BINDING PROTEINS; TRANSLATIONAL INHIBITION; FLOWERING-TIME; PRI-MIRNA; GENES; SERRATE;

D O I：

10.1073/pnas.1012891107

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In Arabidopsis thaliana, four different dicer-like (DCL) proteins have distinct but partially overlapping functions in the biogenesis of microRNAs (miRNAs) and siRNAs from longer, noncoding precursor RNAs. To analyze the impact of different components of the small RNA biogenesis machinery on the transcriptome, we subjected dcl and other mutants impaired in small RNA biogenesis to whole-genome tiling array analysis. We compared both protein-coding genes and noncoding transcripts, including most pri-miRNAs, in two tissues and several stress conditions. Our analysis revealed a surprising number of common targets in dcl1 and dcl2 dcl3 dcl4 triple mutants. Furthermore, our results suggest that the DCL1 is not only involved in miRNA action but also contributes to silencing of a subset of transposons, apparently through an effect on DNA methylation.

引用

页码：17466 / 17473

页数：8

共 84 条

[41] Biochemical Evidence for Translational Repression by Arabidopsis MicroRNAs [J].