Polymer-based elemental tags for sensitive Bioassays

被引：259

作者：

Lou, Xudong

Zhang, Guohua

Herrera, Isaac

Kinach, Robert

Ornatsky, Olga

Baranov, Vladimir

Nitz, Mark

Winnik, Mitchell A.

机构：

[1] Univ Toronto, Inst Biomat & Biomed Engn, Toronto, ON M5S 3G9, Canada

[2] Univ Toronto, Dept Chem, Toronto, ON M5S 3H6, Canada

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 32期

关键词：

antibodies; bioassays; chelates; mass spectrometry; polymers;

D O I：

10.1002/anie.200700796

中图分类号：

O6 [化学];

学科分类号：

0703 [化学];

摘要：

(Figure Presented) It's elemental: A water-soluble polymer bearing multiple metal-chelating ligands has been used as a tag for bioassays with inductively coupled plasma mass spectrometry. The tag was covalently conjugated to antibodies, and the polymer-antibody constructs were loaded with lanthanide ions (Ln3+) and used for the simultaneous assay of five orthogonally labeled antibodies against cell surface antigens that differ in abundance by more than two orders of magnitude. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

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收藏

页码：6111 / 6114

页数：4

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共 15 条

[1]

Lanthanide(III) complexes of DOTA-glycoconjugates:: A potential new class of lectin-mediated medical imaging agents [J].

André, JP ;

Geraldes, CFGC ;

Martins, JA ;

Merbach, AE ;

Prata, MIM ;

Santos, AC ;

de Lima, JJP ;

Tóth, É .

CHEMISTRY-A EUROPEAN JOURNAL, 2004, 10 (22) :5804-5816

[2]

The potential for elemental analysis in biotechnology [J].

Baranov, VI ;

Quinn, ZA ;

Bandura, DR ;

Tanner, SD .

JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 2002, 17 (09) :1148-1152

[3]

A sensitive and quantitative element-tagged immunoassay with ICPMS detection [J].

Baranov, VI ;

Quinn, Z ;

Bandura, DR ;

Tanner, SD .

ANALYTICAL CHEMISTRY, 2002, 74 (07) :1629-1636

[4]

DNA-mediated electron transfer: Chemistry at a distance [J].

Barton, JK .

PURE AND APPLIED CHEMISTRY, 1998, 70 (04) :873-879

[5]

The case for early detection [J].

Etzioni, R ;

Urban, N ;

Ramsey, S ;

McIntosh, M ;

Schwartz, S ;

Reid, B ;

Radich, J ;

Anderson, G ;

Hartwell, L .

NATURE REVIEWS CANCER, 2003, 3 (04) :243-252

[6]

Proteomics in multiplex [J].

Melton, L .

NATURE, 2004, 429 (6987) :101-+

[7]

Polymer terminal group effects on properties of thermoresponsive polymeric micelles with controlled outer-shell chain lengths [J].

Nakayama, M ;

Okano, T .

BIOMACROMOLECULES, 2005, 6 (04) :2320-2327

[8]

Multiple cellular antigen detection by ICP-MS [J].

Ornatsky, O ;

Baranov, V ;

Bandura, DR ;

Tanner, SD ;

Dick, J .

JOURNAL OF IMMUNOLOGICAL METHODS, 2006, 308 (1-2) :68-76

[9]

Being excited by lanthanide coordination complexes: Aqua species, chirality, excited-state chemistry, and exchange dynamics [J].

Parker, D ;

Dickins, RS ;

Puschmann, H ;

Crossland, C ;

Howard, JAK .

CHEMICAL REVIEWS, 2002, 102 (06) :1977-2010

[10]

Innovation - Seventeen-colour flow cytometry: unravelling the immune system [J].

Perfetto, SP ;

Chattopadhyay, PK ;

Roederer, M .

NATURE REVIEWS IMMUNOLOGY, 2004, 4 (08) :648-U5