Application of high-speed counter-current chromatography to the separation of coumarin and related compounds

被引：10

作者：

Shibusawa, Y ^{[1
]}

Hagiwara, Y ^{[1
]}

Chao, ZM ^{[1
]}

Ma, Y ^{[1
]}

Ito, Y ^{[1
]}

机构：

[1] NHLBI,BIOPHYS CHEM LAB,NIH,BETHESDA,MD 20892

来源：

JOURNAL OF CHROMATOGRAPHY A | 1997年 / 759卷 / 1-2期

基金：

美国国家卫生研究院;

关键词：

counter-current chromatography; coumarin; hydroxycinnamic acids; esculin; organic acids;

D O I：

10.1016/S0021-9673(96)00762-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Coumarin and related compounds were successfully separated by high-speed counter-current chromatography (CCC). Using the conventional technique with the cross-axis coil planet centrifuge, coumarin, esculin, 2- and 3- or 4-hydroxycinnamic acids were well resolved with a solvent system composed of ethyl acetate-10 mM potassium phosphate (1:1, v/v) at pH 6.5, while the separation between 3- and 4-hydroxycinnamic acids was difficult. The separation of 2-, 3- and 4-hydroxycinnamic acids, was achieved by pH-zone-refining CCC using a type-J multilayer coil planet centrifuge with a methyl-tert.-butyl ether-acetonitrile-water (1:0:1, or 4:1:5, v/v) system where trifluoroacetic acid was added to the organic mobile phase as an eluter and while ammonia was added to the aqueous stationary phase as a retainer.

引用

页码：47 / 53

页数：7

共 11 条

[1] IMPROVED CROSS-AXIS SYNCHRONOUS FLOW-THROUGH COIL PLANET CENTRIFUGE FOR PERFORMING COUNTERCURRENT CHROMATOGRAPHY .2. STUDIES ON RETENTION OF STATIONARY PHASE IN SHORT COILS AND PREPARATIVE SEPARATIONS IN MULTILAYER COILS [J].