Screening, confirmation and quantitation of diuretics in urine for doping control analysis by high-performance liquid chromatography-atmospheric pressure ionisation tandem mass spectrometry

被引：62

作者：

Thieme, D ^{[1
]}

Grosse, J ^{[1
]}

Lang, R ^{[1
]}

Mueller, RK ^{[1
]}

Wahl, A ^{[1
]}

机构：

[1] Inst Doping Anal & Sports Biochem, D-01731 Kreischa, Germany

来源：

JOURNAL OF CHROMATOGRAPHY B | 2001年 / 757卷 / 01期

关键词：

doping control; diuretics; benzothiadiazine;

D O I：

10.1016/S0378-4347(01)00058-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive, selective, robust and fast method to identify 32 diuretics and masking agents in mine is described. The analytical procedure is reduced to a single XAD extraction step for sample preparation, followed by reversed-phase liquid chromatography in combination with atmospheric pressure ionisation/tandem mass spectrometry. This technique is, after minor modifications, suitable for screening analyses and confirmation of identity as well as quantitation of diuretics. Considerations relating to the stability and metabolism of the compounds are given if relevant for routine screening analyses. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：49 / 57

页数：9

共 20 条

[11]

*IOC, CRIT REP LOW CONC AN

[12]

IOC (International Olympic Committee), 2000, OL MOV ANT COD, P49

[13] SCREENING FOR DIURETICS IN URINE AND BLOOD-SERUM BY CAPILLARY ZONE ELECTROPHORESIS [J].

JUMPPANEN, J ;

SIREN, H ;

RIEKKOLA, ML .

JOURNAL OF CHROMATOGRAPHY A, 1993, 652 (02) :441-450

[14] Liquid chromatography mass spectrometry in forensic and clinical toxicology [J].

Maurer, HH .

JOURNAL OF CHROMATOGRAPHY B, 1998, 713 (01) :3-25

[15] A COLUMN-SWITCHING TECHNIQUE FOR THE SCREENING OF DIURETICS IN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

SAARINEN, M ;

SIREN, H ;

RIEKKOLA, ML .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1993, 16 (18) :4063-4078

[16] Derivatization procedures for gas chromatographic mass spectrometric determination of xenobiotics in biological samples, with special attention to drugs of abuse and doping agents [J].

Segura, J ;

Ventura, R ;

Jurado, C .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1998, 713 (01) :61-90

[17]

THIEME D, 2000, RECENT ADV DOPING AN, V8

[18] Detection of diuretic agents in doping control [J].

Ventura, R ;

Segura, J .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1996, 687 (01) :127-144

[19] APPROACH TO THE ANALYSIS OF DIURETICS AND MASKING AGENTS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY IN DOPING CONTROL [J].

VENTURA, R ;

FRAISSE, D ;

BECCHI, M ;

PAISSE, O ;

SEGURA, J .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1991, 562 (1-2) :723-736

[20] Identification of lorazepam and sildenafil as examples for the application of LC/ionspray-MS and MS-MS with mass spectra library searching in forensic toxicology [J].

Weinmann, W ;

Lehmann, N ;

Müller, C ;

Wiedemann, A ;

Svoboda, M .

FORENSIC SCIENCE INTERNATIONAL, 2000, 113 (1-3) :339-344

← 1 2 →