Pancreatic lipase-related protein 1 (PLRP1) is present in the pancreatic juice of several species

被引：45

作者：

De Caro, J

Carrière, F

Barboni, P

Giller, T

Verger, R

De Caro, A

机构：

[1] CNRS, UPR 9025, IFRC 1, Lipolyse Enzymat Lab, F-13402 Marseille 20, France

[2] F Hoffmann La Roche & Co Ltd, CH-4002 Basel, Switzerland

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1998年 / 1387卷 / 1-2期

关键词：

pancreatic juice; pancreatic lipase-related protein; baculovirus expression system; (man); (dog); (pig); (rat);

D O I：

10.1016/S0167-4838(98)00143-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pancreatic lipase-related protein 1 (PLRP1) was purified from human, canine, porcine and rat pancreatic juices. The four PLRPls were identified using microsequencing methods after performing gel filtration on Ultrogel AcA-54 followed by chromatography on Heparin-Sepharose cation-exchanger. Polyclonal antibodies specific to human PLRP1 (HPLRP1) were raised in the rabbit using a synthetic decapeptide from HPLRP1. The results of Western blotting analysis showed that these antibodies recognized native HPLRP1 and recombinant HPLRP1 produced by insect cells, and cross-reacted only with rat PLRP1 (RPLRP1). No significant lipolytic activity was observed with native canine PLRP1 and recombinant HPLRP1 on various glycerides, phospholipid and vitamin esters, or on cholesterol esters. It was established for the first time that this protein is secreted in variable amounts by the adult exocrine pancreas of several species. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：331 / 341

页数：11

共 40 条

[31] Sambrook J., 1989, MOL CLONING
[32] THE HUMAN PANCREATIC LIPASE-ENCODING GENE - STRUCTURE AND CONSERVATION OF AN ALU SEQUENCE IN THE LIPASE GENE FAMILY
SIMS, HF
JENNENS, ML
LOWE, ME
[J]. GENE, 1993, 131 (02) : 281 - 285
[33] SPARKES R S, 1987, Genomics, V1, P138, DOI 10.1016/0888-7543(87)90005-X
[34] ONE-STEP PURIFICATION AND CHARACTERIZATION OF HUMAN PANCREATIC LIPASE EXPRESSED IN INSECT CELLS
THIRSTRUP, K
CARRIERE, F
HJORTH, S
RASMUSSEN, PB
WOLDIKE, H
NIELSEN, PF
THIM, L
[J]. FEBS LETTERS, 1993, 327 (01) : 79 - 84
[35] EVIDENCE FOR A PANCREATIC LIPASE SUBFAMILY WITH NEW KINETIC-PROPERTIES
THIRSTRUP, K
VERGER, R
CARRIERE, F
[J]. BIOCHEMISTRY, 1994, 33 (10) : 2748 - 2756
[36] WAGNER ACC, 1994, J BIOL CHEM, V269, P9099
[37] WARDEN CH, 1993, J LIPID RES, V34, P1451
[38] PRIMARY STRUCTURE OF RAT PANCREATIC LIPASE MESSENGER-RNA
WICKERPLANQUART, C
PUIGSERVER, A
[J]. FEBS LETTERS, 1992, 296 (01) : 61 - 66
[39] WISHART MJ, 1993, J BIOL CHEM, V268, P10303
[40] A pancreatic lipase with a phospholipase A1 activity: Crystal structure of a chimeric pancreatic lipase-related protein 2 from guinea pig
WithersMartinez, C
Carriere, F
Verger, R
Bourgeois, D
Cambillau, C
[J]. STRUCTURE, 1996, 4 (11) : 1363 - 1374

← 1 2 3 4 →