DNA-Cu(II) complex as a novel electrocatalyst for a hydrogen peroxide sensor

被引：34

作者：

Hasebe, Y

Gu, TT

机构：

[1] Saitama Inst Technol, Dept Appl Chem, Okabe, Saitama 3690293, Japan

[2] Saitama Inst Technol, Grad Sch Engn, Dept Mat Sci & Engn, Okabe, Saitama 3690293, Japan

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 2005年 / 576卷 / 01期

关键词：

DNA-Cu(II) complex; electrocatalyst; DNA/polyamine membrane; hydrogen peroxide;

D O I：

10.1016/j.jelechem.2004.10.010

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A DNA-Cu(II) complex was effectively utilized as a novel electrocatalyst for an H2O2-sensing electrode. The DNA-Cu(II) complex was immobilized onto the surface of a glassy carbon (GC) electrode by polyion complex formation with poly(allylamine) (PAA). Cyclic voltammetry revealed that the copper ion embedded in the DNA/PAA layer exhibited a pair of well-defined redox peaks which is due to the Cu(II)/Cu(I) redox couple. The resulting DNA-Cu(II)/PAA/GC electrode showed excellent electrocatalytic activity for H2O2 reduction. The amperometric response to H2O2 obtained at -0.2 V (vs. Ag\AgCl) was rapid and highly sensitive. A calibration curve obtained in air-saturated buffer (pH 5) was linear in the concentration range from 0.1 to 135 muM with a detection limit of 0.05 muM (S/N = ca. 3). Moreover, this sensor showed long term-storage stability of more than 30 days under dry conditions 4 degreesC. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：177 / 181

页数：5

共 23 条

[11]

LUAN C, 1988, ANAL CHEM, V70, P1721

[12]

LUAN C, 1998, J ELECTROANAL CHEM, V455, P121

[13] AMPEROMETRIC L-LACTATE-SENSING ELECTRODE BASED ON A POLYION COMPLEX LAYER CONTAINING LACTATE OXIDASE - APPLICATION TO SERUM AND MILK SAMPLES [J].